J Rhinol.  2006 May;13(1):32-36.

Inhibition of Fungi-Induced Nasal Polyp Epithelial Cells Activation by Corticosteroids

• Shin SH
• Ye MK

Abstract

BACKGROUND AND OBJECTIVES: Airway epithelial cells contribute to the pathogenesis of air disease by their interaction with inhalant pathogenic extracts. Airborne fungi interact with nasal epithelial cell and enhance the production of inflammatory cytokines. Glucocorticosteroids (GCs) have been used therapeutically for nasal polyps and allergic disease with potent anti-inflammatory effects. The purpose of this study was to investigate the inhibitory effect of GCs on fungi induced nasal epithelial cell activation.
MATERIALS AND METHODS
The epithelial cells of nasal polyps were obtained from patients and stimulated with Alternaria. To evaluate the anti-inflammatory effects of GCs, Alternaria was pretreated with GCs (triamcinolone, dexamethasone, and budesonide) and cultured with epithelial cells. Interleukin-8 (IL-8) and granulocyte-macrophage colony stimulating factor (GM-CSF) were measured to determine the activation of epithelial cells. Reverse transcriptase-polymerase chain reaction (RT-PCR) test for protease-activated receptors (PARs) mRNA expression in nasal epithelial cells were performed.
RESULTS
Alternaria enhanced the production of IL-8 and GM-CSF from nasal epithelial cells. GCs inhibited the activation of nasal epithelial cells, but the PAR2 and PAR3 mRNA expression were not suppressed by GCs.
CONCLUSION
These data suggest that GCs inhibit the production of chemical mediators by Alternaria, but anti-inflammatory effect of GCs are not associated with PARs.

Keyword

Glucocorticosteroid; Alternaria; Cytokine; Protease-activated receptor

MeSH Terms

Adrenal Cortex Hormones*
Alternaria
Colony-Stimulating Factors
Cytokines
Dexamethasone
Epithelial Cells*
Fungi
Granulocyte-Macrophage Colony-Stimulating Factor
Humans
Interleukin-8
Nasal Polyps*
Receptors, Proteinase-Activated
RNA, Messenger
Adrenal Cortex Hormones
Colony-Stimulating Factors
Cytokines
Dexamethasone
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-8
RNA, Messenger
Receptors, Proteinase-Activated