Korean J Urol.  1992 Oct;33(5):771-777.

Analysis of MDR1(multidrug resistance) gene expression in bladder tumor and renal cell carcinoma by polymerase chain reaction

• Kim CS
• Song KY
• Kim KH

Abstract

The expression of the multidrug resistance gene (MDR1, gene) is the main mechanism of tumor resistance to anticancer drugs. MDR1 gene encodes P-glycoprotein which functions as an efflux pump for some anticancer drugs such as vinblastine and doxorubicin. Polymerase chain reaction is the recent technology with allows direct analysis of a unique segment present in the genome of e single cell. We used the new method to analyze the levels of MDR1 mRNA in the tissue samples of kidney, bladder and their tumors, using the polymerase chain reaction. High levels of MDR1 gene expression were revealed in 3 of 4 (75%) normal kidney and in 2of 4 (50%) of renal cell carcinoma tissues. MDR1 gene expression was not observed in 3 normal bladder tissues but low levels of MDR1 gene expression was not observed in 6 of8 (75%) bladder tumor tissues. The polymerase chain reaction may be e highly sensitive, specific, rapid and convenient method in measuring the level of MDR1 gene expression, compared with conventional blot methods.

Keyword

multidrug resistance gene; polymerase chain reaction; bladder tumor; renal cell carcinoma

MeSH Terms

Carcinoma, Renal Cell*
Doxorubicin
Gene Expression*
Genes, MDR
Genome
Kidney
P-Glycoprotein
Polymerase Chain Reaction*
RNA, Messenger
Urinary Bladder Neoplasms*
Urinary Bladder*
Vinblastine
Doxorubicin
P-Glycoprotein
RNA, Messenger
Vinblastine