J Korean Orthop Assoc.  2002 Jun;37(3):411-415.

Changes of Serum Alkaline Phosphatase and Osteocalcin during Fracture Healing

• Lee HS
• Lee CS
• Jang JS
• Lee JD
• Um SM

Abstract

PURPOSE: To investigate changes of serum osteoblastic marker during fracture healing.
MATERIALS AND METHODS
The study included 22 patients with fresh fractures. Serum alkaline phosphatase (ALP), bone specific alkaline phosphatase and osteocalcin were analyzed on the first day and 1, 2, 4 and 8 weeks after injury. The bone specific-ALP was quantified by electrophoresis. Osteocalcin was quantified by enzyme linked immunosorbent assay (ELISA).
RESULTS
One, 2, 4 and 8 weeks after injury, mean values of serum ALP increased 1.26, 1.45, 1.63, and 1.2 times that on the first day after injury, respectively. Similarly, during the same period, bone specific ALP increased 1.38, 1.33, 1.73 and 1.25 times, and serum osteocalcins increased 1.32, 1.2, 1.64 and 2.09 times.
CONCLUSION
Serum ALP increased during the early soft callus phase. However, serum osteocalcins increased during the late hard callus phase. Serum alkaline phosphatase and osteocalcin warrant further study as useful prognostic indicators of fracture healing.

Keyword

Fracture healing; Osteoblastic marker; Alkaline-phosphatase; Osteocalcin

MeSH Terms

Alkaline Phosphatase*
Bony Callus
Electrophoresis
Enzyme-Linked Immunosorbent Assay
Fracture Healing*
Humans
Osteoblasts
Osteocalcin*
Alkaline Phosphatase
Osteocalcin