J Korean Soc Coloproctol.  2009 Feb;25(1):26-33. 10.3393/jksc.2009.25.1.26.

The Expression and Clinical Correlations of Matrix Metalloproteinase-2, -7, -9, and -12 in Colorectal Cancer

• Ahn EJ
• Chung SS
• Lee RA
• Kim KH

• KMID: 2101469
• DOI: http://doi.org/10.3393/jksc.2009.25.1.26

Abstract

PURPOSE: Tumor invasion and metastasis are multistep phenomina, involving proteolytic degradation of extracellular matrix (ECM) and alteration of cell adhesion. It is another oncologic therapeutic strategy to block tumor invasion and metastasis through analyzing the molecules which are involved in these processes. We examined the expressions of some of matrix metalloproteinases (MMPs) in colorectal cancer and analyze the correlation with clinical factors and survival.
METHODS
Fifty-five patients with colorectal cancer who underwent surgical resection were included. The expressions of the MMP-2, -7, -9, and -12 in tumor tissue and normal mucosa using RT-PCR technique was carried out. We evaluated and analyzed the correlation among these molecules, clinical characteristics, and survival.
RESULTS
Expressions of MMP-7, -9, and -12 were significantly higher in tumor tissue than in normal mucosa (P=0.00). The expressions of MMP-2 between cancer and normal mucosa had no significant difference but it had a significant difference according to the lymph node (LN) invasion (P=0.03) in tumor tissues. Three-year overall survival was worse in patient with high expression of MMP-2 compared to low expression.
CONCLUSION
The expressions of MMP-7, -9, -12 in tumor tissue were higher than in normal mucosa and MMP-2 expression of tumor tissues had a significant difference according to LN invasion. MMP-2 overexpression seems to be a prognostic factor for 3-yr overall survival. But, large scale study with long term survival analysis will be needed for the prognostic significance of MMPs.

Keyword

Matrix metalloproteinase; Colorectal cancer

MeSH Terms

Cell Adhesion
Colorectal Neoplasms
Extracellular Matrix
Humans
Lymph Nodes
Matrix Metalloproteinase 2
Matrix Metalloproteinases
Mucous Membrane
Neoplasm Metastasis
Matrix Metalloproteinase 2
Matrix Metalloproteinases