J Korean Soc Coloproctol.  1998 Mar;14(1):21-26.

A Preliminary Study on the Expression and Regulation of C-X-C Chemokine Genes in a Human Colon Epithelial Cell Line

• Yang SK
• Kim OH
• Lee MH
• Park SM
• Jung HY
• Hong WS
• Min YI

Abstract

Human colon epithelial cells secrete an array of proinflammatory cytokines that includes IL-8, MCP-1, GM-CSF, TNF alpha and IL-6. This response may serve to attract neutrophils and macrophags to the site of infection. In addition to IL-8 and MCP-1, the chemokine family contains other members, which, alone or in combination, can recruit and/or activate inflammatory and lymphoid cells. In this study, we asked whether colon epithelial cells express a broader array of chemokines than previously described. The colon epithelial cell line, Caco-2, was stimulated for 3h with IL-1 alpha, or was infected with Salmonella dublin. RNA was extracted and chemokine mRNA levels were determined by quantitative reverse transcription-PCR using internal RNA standards. Ex pression of GRO alpha, GRO beta, GRO gamma and IP-10 increased by bacterial infection or IL-l alpha stimulation. These data strongly support the notion that epithelal cells are an important and integral component of the host's natural immune system.

Keyword

Chemokine; Epithelial cell; Colon

MeSH Terms

Bacterial Infections
Chemokines
Colon*
Cytokines
Epithelial Cells*
Granulocyte-Macrophage Colony-Stimulating Factor
Humans*
Immune System
Interleukin-1alpha
Interleukin-6
Interleukin-8
Lymphocytes
Neutrophils
RNA
RNA, Messenger
Salmonella
Chemokines
Cytokines
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-1alpha
Interleukin-6
Interleukin-8
RNA
RNA, Messenger