J Korean Soc Plast Reconstr Surg.  2006 Jul;33(4):413-422.

The Effect of Silk Fibroin-Alginic Acid Sponge Treatment as an Artificial Skin on Wound

• Oh M
• Choe J
• Heo CY
• Baik LM
• Kim YS
• Choi YW

Abstract

PURPOSE
This study was designed to compare the wound healing effect of silk fibroin, alginate and fibroin/ alginate blend sponge with clinically used Nu gauze in a rat skin defect model.
METHODS
Two full thickness excisions were made on the back of Sprague-Dawley rat. The excised wound was covered with either of the silk fibroin(SF), alginate (SA), or fibroin/alginate blend sponge(SF/SA). On the postoperative days of 3, 7, 10 and 14, the wound area was calculated by image analysis software. At the same time, a skin wound tissue was biopsied.
RESULTS
Healing time 50% (HT(50)) of SF/SA sponge treated group was dramatically reduced as compared with that of control treatment. We also found that the HT50 of SF/SA sponge was significantly decreased as compared with either those of SF or SA treatment. Furthermore, SF/SA treatment significantly increased the size of epithelialization and collagen deposition as well as the number of PCNA positive cells on epidermal basement membrane as comapred with those of control treatment.
CONCLUSION
Our results suggest that the wound healing effect of SF/SA blend sponge is the best among other treatments including SF and SA during the whole wound healing period.

Keyword

Silk fibroin-alginic acid; Wound healing; Epithelialization; PCNA

MeSH Terms

Animals
Basement Membrane
Collagen
Fibroins
Porifera*
Proliferating Cell Nuclear Antigen
Rats
Rats, Sprague-Dawley
Silk*
Skin
Skin, Artificial*
Wound Healing
Wounds and Injuries*
Collagen
Fibroins
Proliferating Cell Nuclear Antigen
Silk