Evaluation of DNA Double Strand Breaks in Human and Mouse Lymphocyte Following gamma-Irradiation

Kim, Tae Hwan; Kim, Sung Ho; Chung, In Yong; Cho, Chul Koo; Koh, Kyung Hwan; Yoo, Seong Yul

Abstract

The evaluation of radiation-induced DNA double strand breaks(DSB) was made following irradiation of human lymphocytes, murille lymphocytes and EL-4 leukemia cells over a wide dose range of 60Co g-rays. In lipopolysacchande(LPS) or phytohemagglutinin(PHA)-stimulated murine lymphocytes, the slopes of the strand scission factor(SSF) revealed that lymphocytes with LPS increased DNA DSB formation by a factor of 1.432 (p<0.005). Furthermore, strand break production was relatively inefficient in the T lymphocytes compared to the B lymphocytes. And EL-4 leukemia cells were found to form significantly more DNA DSB to a greater extent than normal lymphocytes (p<0.705). The in vitro studies of the intrinsic radiosensitivity between human lymphocytes and murine lymphocytes showed similar phasic kinetics. However, murine lymphocytes were lower in DNA DSB formation and higher in the relative radiation dose of 10 percent DNA strand breaks at 3.5 hours following g-irradiation than human lymphocytes. Though it is difficult to interpret these results, these differences may be result from environmental and genetic factors. From our data, if complementary explanations for this difference will be proposed, the differences in the dose-effect relationship for the induction of DSB between humans and mice must be related to interspecies variations in the physiological condition of the peripheral blood in vitro and not to differences in the intrinsic radiation sensitivity of the lymphocytes. These results can be estimated on the basis of dose-effect correlation enabling the interpretation of clinical response and the radiobiological parameters of cytometrical assessment.