J Korean Soc Ther Radiol.  1990 Dec;8(2):137-144.

Quantitative Analysis of DNA Single-strand Breaks in EL 4 cells and Mouse Spleen Lymphocytes after Irradiation

• Yoo SY
• Cho CK
• Koh KH
• Park WY
• Park YH
• Kim SH
• Kim TH
• Chung IY

Abstract

The filter elution technique was used to assay Co-60 g ray-induced DNA single-strand breaks (SSB) in EL 4 mouse leukemia cell and spleen lymphocyte. The lymphocytes were stimulated with lipopolysaccharide (LPS, 20 mug/ml) to label [3H] thymidine. EL 4 cells and lymphocytes in suspension were exposed at 0degree C to gy, 1 gy, 5 gy, 10 gy of Co-60 radiation and elution procedure was performed at PH 12.1. The number of DNA single-strand breaks increased with increasing doses of g rays. The strand scission factor (SSF) was estimated in each experiment (eluted volume 21 ml. The slope for EL 4 cells was 0.01301+/-0.00096 gy-1(n=5) and the slope for lymphocytes was 0.01097+/-0.00091 gy-1(n=5). The slopes were significantly different (p<0.005). Thus EL 4 cells were more sensitive to induction of DNA SSB by ionizing radiation than lymphocytes.

Keyword

Alkaline elution; EL 4; Lymphocytes; Single strand breaks; Filter

MeSH Terms

Animals
DNA*
Hydrogen-Ion Concentration
Leukemia
Lymphocytes*
Mice*
Radiation, Ionizing
Spleen*
Thymidine
DNA
Thymidine