Korean J Pediatr Gastroenterol Nutr.  1998 Sep;1(1):90-99.

A Study of Serum Cytokines in the Patients with Chronic Hepatitis B Virus Infection

• Kim BJ
• Ma JS
• Hwang TJ

Abstract

PURPOSE: The aim of this study was to clarify the serum cytokine pattern in patients with chronic HBV infection in terms of their clinical state.
METHODS
Intravenous blood samples were taken from 35 patients who were seropositive for HBsAg for at least 6 months and 7 healthy controls. Samples were initially tested for serum aminotransferases and serologic markers for hepatitis B virus by EIA. Serum levels of interleukin(IL)-2, tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), IL-4, and IL-10 were measured by ELISA.
RESULTS
Among 35 patients, seropositive for HBeAg was 20 and for anti-HBe was 15. The histologic diagnosis of 19 patients underwent liver biopsy were chronic persistent hepatitis (CPH) in 10 and chronic active hepatitis (CAH) in 9. Serum IL-10 level in patients seropositive for HBeAg was significantly higher than that in patients seropositive for anti-HBe (p<0.05). All measured cytokine levels in patients with CAH were higher than those of patients with CPH. High values of all measured cytokines except IL-4 were seen in patients with AST and ALT > 100 U/L. High level of IL-4 was seen in patients with normal aminotransferase levels.
Conclusion
These results were thought to indicate that anti-inflammatory Th2-like cytokine (IL-10) production in chronic HBV infection is related to circulating HBeAg rather than activity of hepatitis and that Th1 cytokines seem to be associated with the increasing activity of hepatitis.

Keyword

Serum cytokine; Chronic hepatitis B infection

MeSH Terms

Biopsy
Cytokines*
Diagnosis
Enzyme-Linked Immunosorbent Assay
Hepatitis
Hepatitis B e Antigens
Hepatitis B Surface Antigens
Hepatitis B virus
Hepatitis B, Chronic*
Hepatitis, Chronic*
Humans
Interferon-gamma
Interleukin-10
Interleukin-4
Liver
Transaminases
Tumor Necrosis Factor-alpha
Cytokines
Hepatitis B Surface Antigens
Hepatitis B e Antigens
Interferon-gamma
Interleukin-10
Interleukin-4
Transaminases
Tumor Necrosis Factor-alpha