Korean J Pediatr Hematol Oncol.
1999 Apr;6(1):105-114.
Establishment of Cord Blood Stem Cell Bank in Korea
- Affiliations
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- 1Department of Pediatrics, Chungnam National University College of Medicine, Korea.
- 2Department of Pediatrics, Kangwon National University College of Medicine, Korea.
- 3Department of Pediatrics, Chungbuk National University, College of Medicine, Korea.
- 4Department of Pediatrics, Sung Kyun Kwan University College of Medicine, Korea.
- 5Department of Pediatrics, Catholic University College of Medicine, Korea.
- 6Department of Pediatrics, Chonnam National University, College of Medicine, Korea.
- 7Department of Pediatrics, Ewha Womans University College of Medicine, Korea.
- 8Department of Pediatrics, Dong-A University College of Medicine, Korea.
- 9Department of Pediatrics, Seoul National University, College of Medicine, Korea. hyshin@plaza.snu.ac.kr
- 10Department of Pediatrics, Keimyung University College of Medicine, Korea.
- 11Department of Pediatrics, Kyungpook National University College of Medicine, Korea.
- 12Department of Pediatrics, Kyung Hee University College of Medicine, Korea.
Abstract
- PURPOSE: The needs of stem cell transplantation are increasing in Korea under the circumstance of medical insurance coverage since 1996. But allogeneic bone marrow transplants have the limitation in acquisition of matched donor because of small number of siblings in modern society. Although unrelated stem cell transplantations were made possible in Korea with the establishment of Korean Marrow Donor Program, less than one third of patients who need stem cell transplantation are seeking the matched donors through this program and it still takes too much time and efforts. In this clinical setting, cord blood offers an exciting alternative to traditional sources of stem cells. To implement the widespread clinical use of cord blood stem cells, it is necessary to establish the cord blood bank from which stem cell specimens are obtained.
METHODS
To establish the cord blood bank in Korea, we started to develop the proper methods to obtain the cord bloods. Various ways of collecting the cord bloods, separation of red blood cells and storage methods were tried and the standard recommendations were made.
RESULTS
Mean volume of collected umbilical cord blood was above 70mL by open or closed method in vaginal delivery and cesarean section. Among the RBC depletion methods by using 3% gelatin, Ficoll-Hypaque, or modified Ficoll-Hypaque method, 3% gelatin sedimentation showed the best result in viability and number of mononuclear cells(MNC), CD34+ cell, and day 14 CFU-GM. The recovery rate after freezing-thawing MNC was higher in the group of using 10% DMSO and programmed freezer starting from 4degrees C than in the group of using mixed with DMSO and hydroxyethyl starch and without programmed freezer. Nine patients underwent cord blood transplantation until December 1998. Among them, 6 patients showed the long-term successful engraftment.
CONCLUSION
We have developed simple, efficient, and reproducible methods for collection, processing, and storage of cord blood to establish the cord blood bank. There are some administrative issues including the legal and financial problems that possibly will be resolved by the support of the government and the medical team.
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