Korean J Physiol Pharmacol.  2015 Jan;19(1):59-64. 10.4196/kjpp.2015.19.1.59.

Pectin Micro- and Nano-capsules of Retinyl Palmitate as Cosmeceutical Carriers for Stabilized Skin Transport

• Ro J
• Kim Y
• Kim H
• Park K
• Lee KE
• Khadka P
• Yun G
• Park J
• Chang ST
• Lee J
• Jeong JH
• Lee J

• KMID: 2071820
• DOI: http://doi.org/10.4196/kjpp.2015.19.1.59

Abstract

Retinyl palmitate (RP)-loaded pectinate micro- and nano-particles (PMP and PNP) were designed for stabilization of RP that is widely used as an anti-wrinkle agent in anti-aging cosmeceuticals. PMP/PNP were prepared with an ionotropic gelation method, and anti-oxidative activity of the particles was measured with a DPPH assay. The stability of RP in the particles along with pectin gel and ethanolic solution was then evaluated. In vitro release and skin permeation studies were performed using Franz diffusion cells. Distribution of RP in each skin tissue (stratum corneum, epidermis, and dermis) was also determined. PMP and PNP could be prepared with mean particle size diameters of 593~843 mum (PMP) and 530 nm (i.e., 0.53 mum, PNP). Anti-oxidative activity of PNP was greater than PMP due largely to larger surface area available for PNP. The stability of RP in PMP and PNP was similar but much greater than RP in pectin bulk gels and ethanolic solution. PMP and PNP showed the abilities to constantly release RP and it could be permeated across the model artificial membrane and rat whole skin. RP was serially deposited throughout the skin layers. This study implies RP loaded PMP and PNP are expected to be advantageous for improved anti-wrinkle effects.

Keyword

Microparticles; Nanoparticles; Pectin; Retinyl palmitate

MeSH Terms

Animals
Diffusion
Epidermis
Ethanol
Gels
Membranes, Artificial
Nanoparticles
Particle Size
Rats
Skin*
Ethanol
Gels
Membranes, Artificial

Figure

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  Fig. 1 Particle size distribution (A: PMP 1 and PMP 2; B: PNP). Data are presented as mean±SD (n=3).

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  Fig. 2 Morphological features of RP-loaded PMP observed with a light microscope at ×40 magnification (A: PMP 1; B: PMP 2). The size bars on the left top in each figure indicate 200 µm.

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  Fig. 3 DPPH radical scavenging effects measured with PMP 1, PMP 2 and PNP. Asterisks (*) indicate a significant difference (p<0.05). Data are presented as mean±SD (n=3).

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  Fig. 4 Changes in remaining percent of RP in formulations tested. Data are presented as mean±SD (n=3).

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  Fig. 5 Release profiles of RP from each formulation tested. Data are presented as mean±SD (n=3).

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  Fig. 6 In vitro skin deposition profiles of RP. Asterisk (*) indicates a significant difference at p<0.05. Data are expressed as mean±SD (n=4).

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