J Clin Pathol Qual Control.  2000 Dec;22(2):283-286.

SDS-Agarose Gel Electrophoresis Patterns of Urine Protein and Serum Protein Electrophoresis Patterns in the Diabetic Nephropathies

• Kwon HJ
• Seo EJ
• Min KO
• Ahn SJ
• Kim BK

Abstract

BACKGROUND: We evaluated serum protein electrophoresis and urine SDS-agarose gel electrophoresis (SDS-AGE) for protein in diabetic nephropathy patients to assess their diagnostic and prognostic value.
METHODS
Fifty four cases of diabetic nephropathy (mean age, 60.9 (1-75) years; male, n=28; female, n=26) and control group (n=10) were studied. Random urine was analyzed by SDS-AGE. These were determined from the presence of low molecular weight and/or high molecular weight proteins by SDS-AGE. Serum protein electrophoresis, total protein, albumin, BUN, creatinine were measured.
RESULTS
The patterns of proteinuria were glomerular (n=11), tubular (n=2), mixed (n=16), mixed-mostly glomerular (n=10), mixed mostly tubular (n=2), albumin only (n=13), Nine cases showed nephrotic syndrome pattern by serum protein electrophoresis. Mean serum total protein was 6.93+/-0.84 g/dL; albumin, 3.91+/-0.56 g/dL; BUN, 26.8+/-17.74 mg/dL; creatinine, 1.52+/-1.02 mg/dL. Compared to the control group, the albumin level of diabetic nephropathies was low, but the alpha 1 and alpha 2 globulin levels were high. A/G ratio was low and beta and gamma fractions showed no differences.
CONCLUSIONS
Using SDS-AGE of proteinuria, various kidney damage patterns were observed. There were no specific findings in diabetic nephropathies by serum protein electrophoresis.

MeSH Terms

Creatinine
Diabetic Nephropathies*
Electrophoresis*
Female
Humans
Kidney
Male
Molecular Weight
Nephrotic Syndrome
Proteinuria
Creatinine