Immune Netw.
2008 Mar;8(1):13-20. 10.4110/in.2008.8.1.13.
Coptis chinensis Extract Inhibits the Production of Inflammatory Mediators and Delayed Type Hypersensitivity in Mice
- Affiliations
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- 1Division of Rheumatology, Department of Internal Medicine, School of Medicine, Kyung Hee University, Korea. yihra@khu.ac.kr
- 2East-West Bone & Joint Research Center, Kyung Hee University East-West Neo Medical Center, Seoul, Korea.
- 3Life Science Research Center, SK Chemicals, Suwon, Korea.
- 4Department of Orthopedic Surgery, College of Medicine, Kyung Hee University, Seoul, Korea.
- KMID: 2054254
- DOI: http://doi.org/10.4110/in.2008.8.1.13
Abstract
- BACKGROUND
Coptis chinensis rhizome has been used as a medicinal herb in traditional Oriental medicine. We investigated the effects of Coptis chinensis extract on inflammatory mediators and delayed type hypersensitivity in mice. METHODS: The inhibitory effect of ethanolic extract of Coptis chinensis (CCE) on cell proliferation was evaluated using MTS assay. The lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and the Con A-activated mouse splenocytes were cultured with various concentrations of CCE. Total nitric oxide (NO) production was determined by Griess reaction. The amounts of secreted prostaglandine E2 (PGE(2)), interleukin (IL)-2 and IFN-gamma were measured by ELISA. To investigate the in vivo anti-inflammatory effect of CCE, oxazolone-induced delayed type hypersensitivity (DTH) model was used. RESULTS: The CCE at 100 microgram/ml significantly blocked the LPS-induced production of pro-inflammatory mediators (NO and PGE) in RAW264.7 macrophages. Also, it significantly inhibited cell proliferation and cytokine (IL-2 and IFN-gamma) production in splenocytes. Furthermore, when splenocytes from CCE fed mice (200 mg/kg for 2 weeks) were activated with Con A, cell proliferation and cytokine production were significantly inhibited. In addition, CCE decreased in vivo inflammation in oxazolone-induced DTH model mice. CONCLUSION: We suggest that Coptis chinensis can be used as an anti-inflammatory drug by exerting an inhibitory effect in inflammatory mediator- and cell-mediated inflammation.
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