Immune Netw.  2003 Dec;3(4):295-301. 10.4110/in.2003.3.4.295.

Induction of CEA-specific Cytotoxic T Lymphocytes by Murine Dendritic Cells Expressing CEA

• Won EH
• Kim CH
• Park MY
• Cho HI
• Oh ST
• Hong YK
• Kim TG

• KMID: 2054234
• DOI: http://doi.org/10.4110/in.2003.3.4.295

Abstract

BACKGROUND
Carcinoembryonic antigen (CEA) is well-known soluble tumor marker frequently detectable in peripheral blood of carcinoma patients and considered as good target for antigen-specific immunotherapy. In this study, we used a replication-deficient adenovirus containing CEA to study CTL induction in vitro after adenovirus-mediated gene transfer into DC. METHODS: DC were obtained from mouse bone marrow and cultured with IL-4 and GM-CSF. For measuring CTL activity, splenocytes were harvested from the mice, which were immunized with DC that had been infected AdV-CEA or pulsed with CEA peptide. Untreated DC was used as a control. Splenocytes were re-stimulated in vitro with DC pulsed with CEA peptide for 7 days and CTL activity with CEA peptide-pulsed EL-4 cells were assessed in a standard 51Cr-release assay. The frequencies of antigen-specific cytokine-secreting T cell were determined with mIFN-gamma ELISPOT. RESULTS: DC infected with recombinant adenovirus expressing CEA induced CEA-specific CTL responses in vivo. Splenocyte induced from mice immunized with AdV-CEA-infected DC increase in the number of IFN-gamma secreting T cells compared with those from mice immunized with CEA peptide-pulsed DC. CONCLUSION: These results suggested that DC infected with recombinant adenovirus has advantages over other forms of vaccination and could provide an alternative approach vaccination therapies.

Keyword

Carcinoembryonic antigen (CEA); adenovirus; dendritic cell (DC); cytotoxic T lymphocytes (CTL)

MeSH Terms

Adenoviridae
Animals
Bone Marrow
Carcinoembryonic Antigen
Dendritic Cells*
Enzyme-Linked Immunospot Assay
Granulocyte-Macrophage Colony-Stimulating Factor
Humans
Immunotherapy
Interleukin-4
Mice
T-Lymphocytes
T-Lymphocytes, Cytotoxic*
Vaccination
Carcinoembryonic Antigen
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-4