Genomics Inform.  2011 Dec;9(4):197-199.

2DSpotDB: A Database for the Annotated Two-dimensional Polyacrylamide Gel Electrophoresis of Pathogen Proteins

Affiliations
  • 1Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Osong 363-951, Korea. junomics@gmail.com

Abstract

The biological interpretation of two-dimensional (2D) gel electrophoresis experiments is a key step toward understanding the functions of biological systems. We here present a web-based integrated database, called 2DSpotDB, for the management of proteome data derived from several pathogens. The 2DSpotDB was established as a part of the management of a pathogen proteome project at the Korea National Institute of Health. The goals of the 2DSpotDB implementation are to store and define important pathogen genes, retrieve information obtained by 2D polyacrylamide gel electrophoresis and mass spectrometry, and create an integrated system to provide pathogen proteome information for biological scientists. This database currently contains 14 gels and information on 387 protein spots, among which 329 proteins were identified and annotated.

Keyword

database; 2D electrophoresis; LC-MS/MS; pathogen proteomics; functional annotation; data mining

MeSH Terms

Acrylic Resins
Data Mining
Electrophoresis
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Gels
Korea
Mass Spectrometry
Proteins
Proteome
Acrylic Resins
Gels
Proteins
Proteome
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