Mycobiology.  2009 Sep;37(3):211-214. 10.4489/MYCO.2009.37.3.211.

Optimal Conditions for Antimicrobial Metabolites Production from a New Streptomyces sp. RUPA-08PR Isolated from Bangladeshi Soil

• Ripa FA
• Nikkon F
• Zaman S
• Khondkar P

• KMID: 2053015
• DOI: http://doi.org/10.4489/MYCO.2009.37.3.211

Abstract

An actinomycete strain was isolated from northern part of Bangladesh and identified as a new Streptomyces species on the basis of its morphological, biochemical, cultural characteristics and 16S rRNA data. Attempts were made to optimize the culture conditions for the production of antimicrobial metabolites by this strain. Antimicrobial metabolites production was started after 7 days of incubation of culture broth and reached its maximum levels after 10 days and thereafter gradually decreased. The maximum production of antimicrobial metabolites was obtained when the culture medium pH was adjusted to 8. The optimum temperature for antimicrobial metabolites production was 39degrees C, indicated the new strain as mesophilic organism. Basel medium supplemented with glucose and yeast extract as carbon and nitrogen sources, respectively, was proved to be the best for the production of bioactive metabolites. Maximum production of bioactive metabolites was when NaCl concentration was 1% and among different minerals tested, K2HPO4 and NaCl showed positive influence on antibiotic production by the strain.

Keyword

Antibacterial activity; Optimization of condition; 16S rRNA; Streptomyces sp.

MeSH Terms

Bangladesh
Carbon
Cultural Characteristics
Glucose
Hydrogen-Ion Concentration
Minerals
Nitrogen
Phosphates
Potassium Compounds
Soil
Sprains and Strains
Streptomyces
Yeasts
Carbon
Glucose
Minerals
Nitrogen
Phosphates
Potassium Compounds
Soil