Mycobiology.  2018 Jun;46(2):138-146. 10.1080/12298093.2018.1468056.

Screening and Evaluation of Streptomyces Species as a Potential Biocontrol Agent against a Wood Decay Fungus, Gloeophyllum trabeum

• Jung SJ
• Kim NK
• Lee DH
• Hong SI
• Lee JK

• KMID: 2428812
• DOI: http://doi.org/10.1080/12298093.2018.1468056

Abstract

Two-hundred and fifty-five strains of actinomycetes isolated from soil samples were screened for their antagonistic activities against four well-known wood decay fungi (WDF), including a brown rot fungus, Gloeophyllum trabeum and three white rot fungi Donkioporia expansa, Trametes versicolor, and Schizophyllum commune. A dual culture assay using culture media supplemented with heated or unheated culture filtrates of selected bacterial strains was used for the detection of their antimicrobial activity against four WDF. It was shown that Streptomyces atratus, S. tsukiyonensis, and Streptomyces sp. greatly inhibited the mycelial growth of the WDF tested compared with the control. To evaluate the biocontrol efficacy of S. atratus, S. tsukiyonensis, and Streptomyces sp., wood blocks of Pinus densiflora inoculated with three selected Streptomyces isolates were tested for weight loss, compression strength (perpendicular or parallel to the grain), bending strength, and chemical component changes. Of these three isolates used, Streptomyces sp. exhibited higher inhibitory activity against WDF, especially G. trabeum, as observed in mechanical and chemical change analyses. Scanning electron microscopy showed that cell walls of the wood block treated with Streptomyces strains were thicker and collapsed to a lesser extent than those of the non-treated control. Taken together, our findings indicate that Streptomyces sp. exhibits the potential to be used as a biocontrol agent for wood decay brown rot fungus that causes severe damage to coniferous woods.

Keyword

Actinomycetes; antagonistic activity; brown rot fungi; white rot fungi

MeSH Terms

Actinobacteria
Cell Wall
Coniferophyta
Culture Media
Fungi*
Hot Temperature
Mass Screening*
Microscopy, Electron, Scanning
Pinus
Schizophyllum
Soil
Streptomyces*
Trametes
Weight Loss
Wood*
Culture Media
Soil