Mycobiology.  2000 Jun;28(2):57-61.

Antibacterial Activity of Water Soluble Components of Elfvingia applanata Alone and in Combinations with Third Generation Cephalosporins

• Eo SK
• Kim YS
• Lee CK
• Lee YN
• Han SS

Abstract

Antibacterial activity of EA, a preparation of water soluble components made from carpophores of Elfvingia applanata (Pers.) Karst, was examined by macrobroth diltution method against a number of bacterial species. Antibacterial effects of EA were expressed as minimal inhibitory concentration (MIC) for growth. Among twelve species of bacteria tested, six strains of each gram positive bacteria and gram negative bacteria, EA showed the most potent antibacterial activity against Staphylococcus epidermidis and Proteus vulgaris, of which MICs were 1.25 mg/ml of EA. To investigate the antibacterial effects of combinations of EA with third generation cepholosporins, such as cefotaxime, ceftriaxone, ceftazidime, and cefixime, the fractional inhibitory concentration (FIC) and fractional inhibitory concentration index (FICI) were determined by macrodilution checkerboard assay for twelve bacterial strains. Combinations of EA and third generation cephalosporins exhibited either additive or indifferent effects in most instances. However, synergistic effects were observed in six instances. No antagonistic effect was observed in any cases.

Keyword

Elfvingia applanata; Antibacterial activity; Macrobroth dilution checkerboard assay; Fractional inhibitory concentration index (FICI)

MeSH Terms

Bacteria
Cefixime
Cefotaxime
Ceftazidime
Ceftriaxone
Cephalosporins*
Gram-Negative Bacteria
Gram-Positive Bacteria
Proteus vulgaris
Staphylococcus epidermidis
Cefixime
Cefotaxime
Ceftazidime
Ceftriaxone
Cephalosporins