Mycobiology.  2012 Jun;40(2):107-110. 10.5941/MYCO.2012.40.2.107.

Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes

• Chukeatirote E
• Maharachchikumbura SSN
• Wongkham S
• Sysouphanthong P
• Phookamsak R
• Hyde KD

• KMID: 2052898
• DOI: http://doi.org/10.5941/MYCO.2012.40.2.107

Abstract

Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template. The cbhI genes were then amplified and cloned using the pGEM-T Easy Vector Systems. The sizes of these PCR amplicons were between 700~800 bp. The DNA sequences obtained were similar showing high identity to the cbhI gene family. These cbhI genes were partial consisting of three coding regions and two introns. The deduced amino acid sequences exhibited significant similarity to those of fungal CBHI enzymes belonging to glycosyl hydrolase family 7.

Keyword

Basidiomycetes; cbh; Cellobiohydrolase; Cellulase; Gene cloning

MeSH Terms

Amino Acid Sequence
Base Sequence
Basidiomycota
Bromides
Cellulase
Cellulose 1,4-beta-Cellobiosidase
Clinical Coding
Clone Cells
Cloning, Organism
DNA
Humans
Introns
Pleurotus
Polymerase Chain Reaction
Quaternary Ammonium Compounds
Sequence Analysis
Bromides
Cellulase
Cellulose 1,4-beta-Cellobiosidase
DNA
Quaternary Ammonium Compounds