Yeungnam Univ J Med.  1995 Dec;12(2):237-245. 10.12701/yujm.1995.12.2.237.

Cloning and Sequencing of the phoA Gene which is Regulated by the phoP-phoQ operon in Pathogenic Enteric Bacteria

• Kim SK
• Lee TY

• KMID: 2320374
• DOI: http://doi.org/10.12701/yujm.1995.12.2.237

Abstract

The DNA fragment containing the phoA of Klebsiella pneumoniae was cloned into pACYC184. The size of the insert. was 4.0 kb and the restriction map showed it contained 3 Pstl sites and 4 PvuLI sites. The nucleotide sequence of the phoA region was determined, which showed strong (80%) sequence similarity with that of Escherichia coli. This suggested that these two species are phylogenetically very close to each other.

Keyword

Klebsiella pneumoniae; phoA gene; Cloning; Sequencing; Sequence similarity

MeSH Terms

Base Sequence
Clone Cells*
Cloning, Organism*
DNA
Enterobacteriaceae*
Escherichia coli
Klebsiella pneumoniae
Operon*
DNA