J Korean Soc Microbiol.
1997 Apr;32(2):151-160.
Cloning and Nucleotide Sequence of the aroA Gene from Shigella sonnei KNIH104S
- Affiliations
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- 1School of Life Sciences, Chungbuk National University, Cheongju, Korea. youngkim@cbucc.chungbuk.ac.kr
- 2Department of Microbiology, National Institute of Health 5 Nokbun-Dong, Eunpyung-Gu, Seoul, Korea.
Abstract
- Shigella sonnei is important causes of human enteric infections. S. sonnei KNIH104S was isolated from patient of shigellosis in Korea and previously reported. We cloned 5.2-kb KpnI fragment containing the aroA gene encoding a 3-enolpyruvylshikimate-5-phosphate synthetase from chromosomal DNA of S. sonnei KNIH104S. This recombinant plasmid was named pSonA 52-1. E. coli CGSC2829, an aroA(-) mutant, cannot grow on the M9 minimal medium but E. coli CGSC2829 (pSonA52-1) can grow on the M9 minimal medium. We sequenced the aroA gene from Shigella for the first time. The aroA gene was composed of 1,284 base pairs with ATG initiation codon and TGA termination codon. Sequence comparison of the aroA gene exhibited 99% identity with that of E. coli. As in the case of E. coli, the serC and aroA genes lie in a single operonic structure.
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