J Korean Soc Microbiol.
1998 Aug;33(4):373-380.
Construction of Genetically Defined aroA Deletion Mutants of Shigella sonnei KNIB104S
- Affiliations
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- 1School of Life Sciences, Chungbuk National University, Cheongju, Korea. youngkim@cbucc.chungbuk.ac.kr
- 2Department of Microbiology, National Institute of Health, Seoul, Korea.
- 3Department of Microbiology, Hannam University, Daejeon, Korea.
Abstract
- In order to develop vaccine candidates against Shigella sonnei KNIH104S, which was isolated in Korea, we have constructed genetically defined aroA deletion mutants using a suicide vector system. They can grow on a chemically defined minimal medium (M9) supplemented with aromatic compounds, but can not grow on a minimal medium without supplements. It was confirmed that the aroA gene had a deletion by PCR technique and Southern hybridization analysis.
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