J Korean Soc Microbiol.
1997 Apr;32(2):161-166.
Cloning of the Form I-antigen Genes from Shigella sonnei KNIH104
- Affiliations
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- 1Department of Microbiology, Chungbuk National University, Cheongju, Korea. jylim@trut.chungbuk.ac.kr
- 2R and D Center, CHEILJEDANG Corporation, Ichon, Korea.
- 3Department of Microbiology, Hannam University, Daejon, Korea.
- 4National Institute of Health, Nokbun-Dong, Eunpyung-Gu, Seoul, Korea.
Abstract
- Shigella sonnei KNIH104S, which was selected by Korean National Institute of Health, expresses form I-antigen as a somatic antigen. In this study, we cloned the genes responsible for form I-antigen synthesis from S. sonnei KNIH104S. A Sau3AI-generated cosmid library of S. sonnei KNIH104S plasmids were transfected into E. coli LE392 and transfectants were tested for agglutination with antiserum against S. sonnei form I-antigen. A clone, JH222, showing the strongest agglutination activity was chosen for further analysis. A recombinant cosmid, pJH222, was isolated from the strain JH222 and retransfected into E. coli LE392. All of the transfectants agglutinated with antiserum against form I-antigen, indicating that pJH222 carried the genes required for S. sonnei form I-antigen synthesis. Restriction analysis of pJH222 revealed a 38 kb insert, which was confirmed by Southern hybridization analysis to be present on a large plasmid of S. sonnei KNIH104S.
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