J Korean Soc Microbiol.  1997 Apr;32(2):161-166.

Cloning of the Form I-antigen Genes from Shigella sonnei KNIH104

Affiliations
  • 1Department of Microbiology, Chungbuk National University, Cheongju, Korea. jylim@trut.chungbuk.ac.kr
  • 2R and D Center, CHEILJEDANG Corporation, Ichon, Korea.
  • 3Department of Microbiology, Hannam University, Daejon, Korea.
  • 4National Institute of Health, Nokbun-Dong, Eunpyung-Gu, Seoul, Korea.

Abstract

Shigella sonnei KNIH104S, which was selected by Korean National Institute of Health, expresses form I-antigen as a somatic antigen. In this study, we cloned the genes responsible for form I-antigen synthesis from S. sonnei KNIH104S. A Sau3AI-generated cosmid library of S. sonnei KNIH104S plasmids were transfected into E. coli LE392 and transfectants were tested for agglutination with antiserum against S. sonnei form I-antigen. A clone, JH222, showing the strongest agglutination activity was chosen for further analysis. A recombinant cosmid, pJH222, was isolated from the strain JH222 and retransfected into E. coli LE392. All of the transfectants agglutinated with antiserum against form I-antigen, indicating that pJH222 carried the genes required for S. sonnei form I-antigen synthesis. Restriction analysis of pJH222 revealed a 38 kb insert, which was confirmed by Southern hybridization analysis to be present on a large plasmid of S. sonnei KNIH104S.

Keyword

Shigella sonnei; form I-antigen genes; large plasmid

MeSH Terms

Agglutination
Clone Cells*
Cloning, Organism*
Cosmids
Plasmids
Shigella sonnei*
Shigella*
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