Nucl Med Mol Imaging.
2008 Oct;42(5):401-409.
Facile Fabrication of Animal-Specific Positioning Molds For Multi-modality Molecular Imaging
- Affiliations
-
- 1Department of Molecular Medicine, Kyungpook National University School of Medicine, Daegu, Korea. yooj@knu.ac.kr
- 2Department of Biomedical engineering, Kyungpook National University, Daegu, Korea.
- 3Department of Radiation Oncology, Kyungpook National University School of Medicine, Daegu, Korea.
- 4Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.
- 5Department of Diagnostic Radiology, Kyungpook National University School of Medicine, Daegu, Korea.
- 6Department of Nuclear Medicine, Kyungpook National University School of Medicine, Daegu, Korea.
Abstract
-
PURPOSE: Recently multi-modal imaging system has become widely adopted in molecular imaging. We tried to fabricate animal-specific positioning molds for PET/MR fusion imaging using easily available molding clay and rapid foam. The animal-specific positioning molds provide immobilization and reproducible positioning of small animal. Herein, we have compared fiber-based molding clay with rapid foam in fabricating the molds of experimental animal.
MATERIALS AND METHODS
The round bottomed-acrylic frame, which fitted into microPET gantry, was prepared at first. The experimental mice was anesthetized and placed on the mold for positioning. Rapid foam and fiber-based clay were used to fabricate the mold. In case of both rapid foam and the clay, the experimental animal needs to be pushed down smoothly into the mold for positioning. However, after the mouse was removed, the fabricated clay needed to be dried completely at 60 degrees C in oven overnight for hardening. Four sealed pipet tips containing [18F]FDG solution were used as fiduciary markers. After injection of [18F]FDG via tail vein, microPET scanning was performed. Successively, MRI scanning was followed in the same animal.
RESULTS
Animal-specific positioning molds were fabricated using rapid foam and fiber-based molding clay for multimodality imaging. Functional and anatomical images were obtained with microPET and MRI, respectively. The fused PET/MR images were obtained using freely available AMIDE program.
CONCLUSION
Animal-specific molds were successfully prepared using easily available rapid foam, molding clay and disposable pipet tips. Thanks to animal-specific molds, fusion images of PET and MR were co-registered with negligible misalignment.
