Tuberc Respir Dis.  1998 Aug;45(4):760-765. 10.4046/trd.1998.45.4.760.

Glucose Transporter Gene Expression in Human Lung Cancer Cell Lines

• Kim WJ
• Yim JJ
• Lee JH
• Yoo CG
• Chung HS
• Han SK
• Chung JK
• Shim YS
• Kim YW

• KMID: 2050092
• DOI: http://doi.org/10.4046/trd.1998.45.4.760

Abstract

BACKGROUND: Glucose uptake has been found to ha increased in cancer cells, and FDG-PET imaging is used for diagnosis of cancer using This phenomenon. However, the exact mechanism of increased glucose uptake in cancer cells has not been clarified. Recent studies demonstrated the presence of glucose transporter(GLUT) mRNA expression in gastrointestinal cancer and bead and neck cancer, and suggested that GLUT may be associated with glucose uptake in cancer cells. In lung cancer cells, glucose metabolism is also known to ha in. creased. We evaluated GLUT mRNA expression in human lung cancer cell lines in order to find out the mechanism of increased glucose uptake in lung cancer. METHOD: Total RNA was isolated from 15 human lung cancer cell lines and immortalized bronchial epithelial cell line(BEAS-2B). After electrophoresis of 20microgram total RNA, Northern blot analysis was done using GLUT1 cDNA and GLUTS cDNA as probes
RESULTS
2 Thirteen of 14 human lung cancer cell lines expressed GLUT1 mRNA and 10 of 14 human lung cancer cell lines expressed GLUT3 mRNA. Eight human lung cancer cell lines expressed both GLUT mRNAs. BEAS-2B expressed GLUT1 mRNA and did not express det~table GLUT3 mRNA. CONCLUISON: The increase of glucose metabolism in lung cancer may be associated with GLUT1 and GLUT3 expression.

Keyword

Glucose transporter(GLUT); Lung neoplasm

MeSH Terms

Blotting, Northern
Cell Line*
Diagnosis
DNA, Complementary
Electrophoresis
Epithelial Cells
Gastrointestinal Neoplasms
Gene Expression*
Glucose Transport Proteins, Facilitative*
Glucose*
Head and Neck Neoplasms
Humans*
Lung Neoplasms*
Lung*
Metabolism
RNA
RNA, Messenger
DNA, Complementary
Glucose
Glucose Transport Proteins, Facilitative
RNA
RNA, Messenger