Korean J Nucl Med.
2000 Aug;34(4):294-302.
The Relationship between F-18-FDG Uptake, Hexokinase Activity and Glut-1 Expression in Various Human Cancer Cell Lines
Abstract
-
PURPOSE: To investigate the mechanisms related to F-18-FDG uptake by tumors, F-18-FDG
accumulation was compared with glucose transporter-1 (Glut-1) expression and hexokinase
activity in various human cancer cell lines.
MATERAL AND METHODS: Human colon cancer (SNU-C2A, SNU-C4, SNU-C5), hepatocellular carcinoma
(SNU-387, SNU-423, SNU-449), lung cancer (NCI-H522, NCI-H358, NCI- H1299), uterine cervical
cancer (HeLa, HeLa 229, HeLa S3) and brain tumor (A172, Hs 683) cell lines were used. After
24 hr incubation of 5x105 cells, 37 kBq F-18-FDG was added and the uptake by cells at 10 min
was measured using a gamma counter. Hexokinase activity was measured by continuous
spectrophotometric rate determination. To measure mitochondrial hexokinase activity,
mitochondrial fraction was separated by a high speed centrifuge. Immunohistochemical
staining of Glut-1 was performed, and graded as 0, 1, 2, or 3 according to expression.
RESULTS
There was difference among F-18-FDG uptake, total and mitochondrial hexokinase activity, and Glut-1 expression with different cancer cell lines. The correlations of F-18-FDG with total hexokinase and mitochondrial hexokinase activity were low (r=0.27 and 0.26, respectively). Glut-1 expression showed a good correlation with F-18-FDG uptake ((p)=0.81, p=0.0015). Previously, we reported no correlation of F-18-FDG uptake with hexokinase activity in colon cancer cell lines. Thus, when colon cancer cells were excluded, F-18-FDG uptake showed higher correlation with total hexokinase and mitochondrial hexokinase activity (r=0.81, p=0.0027 and r=0.81, p=0.0049, respectively).
CONCLUSION
Both Glut-1 expression and hexokinase activity were contributing factors related to F-18-FDG accumulation in human cancer cell lines. The relative contribution of Glut-1 expression and hexokinase activity, however, was different among different cancer cell types.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Mechanisms of Glucose Uptake in Cancer Tissue
- Correlation of GLUT-1 Expression and F-18-FDG Uptake on Positron Emission Tomography in Breast Carcinoma
- Intratumoral Heterogeneous F-18 Fluorodeoxyglucose Uptake Corresponds with Glucose Transporter-1 and Ki-67 Expression in a Case of Krukenberg Tumor: Localization of Intratumoral Hypermetabolic Focus by Fused PET/MR Image
- Glucose Transporter Gene Expression in Human Lung Cancer Cell Lines
- The Ability of FDG Uptake Ratio and Glut-1 Expression to Predict Mediastinal Lymph Node Metastasis in Resected Non-small Cell Lung Cancer
