Lab Med Online.  2015 Oct;5(4):188-195. 10.3343/lmo.2015.5.4.188.

Evaluation of the Automated Hematology Analyzer Sysmex XN-2000 and the Accuracy of Differential Leukocyte Counts Using the Low WBC Mode

• Lee JY
• Song SA
• Oh SH
• Shin JH
• Kim HR
• Jun KR
• Lee JN

• KMID: 2046380
• DOI: http://doi.org/10.3343/lmo.2015.5.4.188

Abstract

BACKGROUND
The XN-series (Sysmex, Japan) is the new hematology analyzer from Sysmex, with new channels to improve the accuracy of differential leukocyte count and platelet count in the low cell count range. We evaluated the analytical performance and low white blood cell (WBC) mode of the XN-2000.
METHODS
Precision, linearity, and carryover were evaluated for the analyzer. We analyzed the accordance of complete blood count (CBC), reticulocyte count, and differential leukocyte count between the XN-2000 and XE-2100 (Sysmex), using 200 samples from normal controls and patients. For 80 samples with a WBC count <1.5x10(9) cells/L, the low WBC mode was evaluated by comparing the automated count with a manual differential count as the reference.
RESULTS
The coefficients of variation of precision were <5% for most CBC parameters and <10% for differential leukocyte count. All results obtained with the XN-2000 showed good correlation with those obtained with the XE-2100. The correlation coefficients (r) were >0.9800 for all CBC parameters except mean corpuscular hemoglobin concentration, mean platelet volume, and platelet distribution width, and >0.9900 for differential leukocyte count except monocytes and basophils. The low WBC mode provided accurate counts for neutrophils and lymphocytes, with r>0.9300 for samples with a WBC count of 0.1-1.5x10(9) cells/L.
CONCLUSIONS
The XN-2000 showed good analytical performance and correlation with the existing model, the XE-2100. The XN-2000 provided accurate results for differential leukocyte count in samples with a WBC count of 0.1-1.5x10(9) cells/L, and reduced manual slide reviews.

Keyword

Hematology analyzer; XN-2000; Differential leukocyte count; Low cell count

MeSH Terms

Basophils
Blood Cell Count
Blood Platelets
Cell Count
Erythrocyte Indices
Hematology*
Humans
Leukocyte Count*
Leukocytes
Lymphocytes
Mean Platelet Volume
Monocytes
Neutrophils
Platelet Count
Reticulocyte Count

Figure

• 

  Fig. 1. Linearities of white blood cell (WBC) count, red blood cell (RBC) count, hemoglobin (Hb), hematocrit (HCT), and platelet (PLT) count determined by the XN-2000 (A, XN-10 B3; B, XN-10 B4).

• 

  Fig. 2. Comparison of complete blood count parameters including mean platelet volume (MPV) and platelet distribution width (PDW) between the XN-2000 and XE-2100 using Bland-Altman plots (A, XN-10 B3; B, XN-10 B4).

Reference

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