J Adv Prosthodont.  2009 Jul;1(2):91-96. 10.4047/jap.2009.1.2.91.

Implant surface treatments affect gene expression of Runx2, osteogenic key marker

• Na Y
• Heo SJ
• Kim SK
• Koak JY

• KMID: 2045469
• DOI: http://doi.org/10.4047/jap.2009.1.2.91

Abstract

STATEMENT OF PROBLEM: The aim of this study was to study the effects of various surface treatments to a titanium surface on the expression of Runx2 in vitro. MATERIAL AND METHODS: Human Osteosarcoma TE-85 cells were cultured on machined, sandblasted, or anodic oxidized cpTi discs. At various times of incubation, the cells were collected and then processed for the analysis of mRNA expression of Runx2 using reverse transcription-PCR. RESULTS: The expression pattern of Runx2 mRNA was differed according to the types of surface treatment. When the cells were cultured on the untreated control culture plates, the gene expression of Runx2 was not increased during the experiments. In the case of that the cells were cultured on the machined cpTI discs, the expression level was intermediate at the first day, but increased constitutively to day 5. In cells on sandblasted cpTi discs, the expression level was highest in the first day sample and the level was maintained to 5 days. In cells on anodized cpTi discs, the expression level increased rapidly to 3 days, but decreased slightly in the 5-th day sample. CONCLUSION: Different surface treatments may contribute to the regulation of osteoblast function by influencing the level of gene expression of key osteogenic factors.

Keyword

Runx2 (Cbfa1); gene expression; implant surface treatment; surface roughness; osteogenesis

MeSH Terms

Durapatite
Gene Expression
Humans
Osteoblasts
Osteogenesis
Osteosarcoma
RNA, Messenger
Titanium
Durapatite
RNA, Messenger
Titanium

Figure

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  Fig. 1 SEM (JSM-840A, JEOL, Japan) of the machined, sandblasted and anodized surface structures of the prepared titanium discs.

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  Fig. 2 Effects of surface treatments on Runx2 mRNA expression in TE-85 cells. Ehidium bromide-stained agarose gel analysis of RT-PCR products. p: control tissue culture plate. m: machined. a: anodic oxidized. s: sandblasted.

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  Fig. 3 a, b. Reverse transcription PCR analysis for Runx2 gene expression by TE-85 cells over time (in days). The amounts and patterns of Runx2 expressions differ in time according to various surface treatments (p: tissue culture plate, m: machined, a: anodic oxidized, s: sandblasted). Values represent Runx2/GAPDH intensity.

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