The Effect of Bone Marrow-Derived Osteoblasts on Mandibular Deffect in Rabbit

Park, Young Ju; Nam, Jeong Hun; Kim, Bo Gyun; Jeon, Min Su; Chung, Jae An; Lee, Jung Won; Ahn, Jang Hoon; Gang, Tae In; Park, Mi Hee; Lim, Sung Chul

Abstract

PURPOSE
The purpose of this experiment was to evaluate the clinical effect of cultured autoglogous osteoblasts as a way to treat the defect of mandible in rabbits.
MATERIALS AND METHODS
Twelve rabbits were used to determine the rate of osteogenesis. The osteoblasts were obtained from the iliac crest of rabbits using aspiration. They were then cultured in Dulbecco's Modified Eagles's Medium (DMEM) with beta-glycerophosophatate, L-ascorbicacid, and dexamethasone to proliferate and differentiate osteoprogenitor cells. The expression of osteogenic markers were detected by reverse transcription-polymerase chain reaction (RT-PCR) and silver nitrate staining techniques. Five, 10-mm holes were placed in each rabbit mandible to simulate defective regions with the use of a low speed trephine bur. In the experimental group, the previously cited defects were grafted with both activated osteoblastic and autogenous bone. The control group, however, was only grafted with autogenous bone. Both groups were then analyzed at 2, 4, and 8-week intervals using bone histomorphometric analysis.
RESULTS
According to histomorphologic analysis, the rates of new bone formation at the 2, 4, and 8-week intervals were 36%, 51%, and 23% for the control group, respectively; 52%, 39%, and 28%, for the experimental group, respectively. The experimental group showed higher rates of new bone formation compared to the control group at both the 2-week and 8-week interval.
CONCLUSION
Bone marrow-derived osteoblasts seems to be a promising bone graft material.