J Korean Orthop Assoc.  1975 Mar;10(1):143-148. 10.4055/jkoa.1975.10.1.143.

Irradiation Effect on the Bone Marrow Regeneration after Homologous Bone Graft

• Kim HS
• Shin YH

• KMID: 2471460
• DOI: http://doi.org/10.4055/jkoa.1975.10.1.143

Abstract

The irradiation is said to have immunosuppressive effect on the allograft and so its effect on the regenerating pattern of the marrow structure following homograft was studied. In normal rabbit the resection of lem long radius was substituted with a homologous radius of same length after 2.000R irradiation of Cs and the graft was immobilized for three weeks with 3cm long intramedullary K-Wire fixation. For ten weeks period after removal of the wire. histological changes of the local medullary cavity were compared with the results obtained with those of autogenous and the homologous graft groups. 1. The autograft (A) group showed that the initial aggregate of the blood clot, the necrotizing tissue, and the inflammatory cellular infiltration almost disappeared at five weeks. and then the cavity was gradually replaced by the fibrous tissue, the fat cells, the new vessels, and the trabeculae, regaining the normal marrow structure after seven or ten weeks. 2. In the fresh homologous graft(H) group, the cellular infiltration was much pronounced and prolonged up to five of seven weeks. Besides the fat cells, the marrow contained the macrophages. the osteoblasts, and the osteoclasts between three and five weeks, and at ten weeks the regeneration of the marrow was incomplete yet. 3. The 2.000 R-irradiated homograft(R) group had, except the less intensive inflammatory cell infiltration, the changes similar to that of the H-group for five weeks. The local medullary cavity. however, was subsequently replaced by proliferation of the fibrous tissue, the developing blood vessels, the fat cells and the osteoblasts. At ten weeks the restitution of the marrow was nearly complete. 4. It follows from the above results that the restitution of the local marrow structure after the homologous graft, when irradiated with 2.000 R of PCs, was prompted favorably and showed histological changes similar to those of the autograft group at ten weeks.

MeSH Terms

Adipocytes
Allografts
Autografts
Blood Vessels
Bone Marrow*
Macrophages
Osteoblasts
Osteoclasts
Radius
Regeneration*
Transplants*

Figure

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  Fig. 1. The blood clot and the fibrous tissue are abundant with mild inflammatory cellular infiltration (one week, autograft). X100

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  Fig. 2. The fibrous tissue, the fat cells and the bizarre cells(bar) are present (three weeks, autograft). X100

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  Fig. 3. Many trabeculae are shown to develop into the medullary cavity(Me) adjacent to the graft bone (Gr), (three weeks, autograft). X100

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  Fig. 4. The fat cells are abundant with the fibrous tissue and the newly developed trabeculae (seven weeks, autograft). X100. Gr: graft bone, Meæ marrow cavity

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  Fig. 5. The marrow structure returns almost to normal (ten weeks, autograft). X200

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  Fig. 6. The trabeculae and the fibrous tissue are developing in the marrow cavity with marked infiltration of the inflammatory cells (three weeks, fresh homograft). X100

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  Fig. 7. The fat cells in the developing marrow cavity are shown (five weeks, homograft). XlOO

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  Fig. 8. The inflammatory cellular infiltration is still moderate (seven weeks, homograft). XlOO

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  Fig. 9. The new marrow cavity contains the fibro-osseous tissue, besides many fat cells (ten weeks, homograft). X100

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  Fig. 10. The inflammatory cells infiltrating diffusely into the fibrous tissue are shown (one week, 2,000 R-irradiated homograft). X100

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  Fig. 11. The fat cells increase in number and the bizarre cells (bar) and a few round cells are shown (five weeks, 2,000 R-irradiated homograft). XlOO

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  Fig. 12. Regeneration of thç bone marrow iş nearly complete (ten weeks, irradiated homograft), X100

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