J Korean Soc Plast Reconstr Surg.  2007 Jul;34(4):426-430.

Effect of Transplantation of Human Bone Marrow Stromal Cells or Dermal Fibroblasts on Wound Healing

• Han SK
• Choi WI
• Lee BI
• Kim WK

Abstract

PURPOSE: The aim of this study is to compare the effects of bone marrow stromal cells(BSCs) and fibroblasts on wound healing activity in vivo, especially on epithelization.
METHODS
The fibroblasts and BSCs were harvested from patients and cultured. Ten Spague-Dawley white rats were used. A 5 mm punches were made to excise skin and subcutaneous tissue in a round fashion at six sites on the back area of each rat. Four hundred thousand cells suspended in 0.05 ml fibrinogen were applied to the created wounds. The cells in group I, II, and III were no cells, fibroblasts and BSCs. The lengths of epithelial gap at the widest wound site were compared with autopsy specimens obtained on the 6th day after cell therapy under light microscope. Statistical comparisons were performed using the Mann-Whitney U-test, and the p value<0.05 was considered statistically significant.
RESULTS
The best epithelization was also seen in the BSC group, followed by fibroblast and no cell groups.
CONCLUSION
These results demonstrate that BSC has superior effect on stimulating wound healing than fibroblast, which is currently used for wound healing.

Keyword

Bone marrow stromal cell; Fibroblast; Wound healing; Epithelization

MeSH Terms

Animals
Autopsy
Bone Marrow*
Cell- and Tissue-Based Therapy
Fibrinogen
Fibroblasts*
Humans*
Mesenchymal Stromal Cells*
Rats
Skin
Subcutaneous Tissue
Wound Healing*
Wounds and Injuries*
Fibrinogen