J Korean Soc Plast Reconstr Surg.  2006 Jan;33(1):1-4.

Comparison of Bone Marrow Stromal Cells with Fibroblasts in Wound Healing Accelerating Growth Factor Secretion

• Kim SH
• Han SK
• Yoon TH
• Kim WK

Abstract

Cryopreserved fibroblast implants represent a major advancement for healing of chronic wounds. Bone marrow stromal cells, which include the mesenchymal stem cells, have a low immunity-assisted rejection and are capable of expanding profoundly in a culture media. Therefore, they have several advantages over fibroblasts in clinical use. The ultimate goal of this study was to compare the wound healing accelerating growth factor secretion of the bone marrow stromal cells with that of the fibroblasts and this pilot study particularly focuses on the growth factor secretion to accelerate wound healing. Bone marrow stromal cells and fibroblasts were isolated from the same patients and grown in culture. At 1, 3, and 5 days post-incubating, secretion of basic fibroblast growth factor(bFGF), vascular endothelial growth factor (VEGF), and transforming growth factor beta(TGF-beta) were compared. In TGF-beta secretion fibroblasts showed 12~21% superior results than bone marrow stromal cells. In contrast, bFGF levels in the bone marrow stromal cells were 47~89% greater than that in fibroblasts. The VEGF levels of the bone marrow stromal cells was 7~12 fold greater than that of the fibroblasts. Our results suggest that the bone marrow stromal cells have great potential for wound healing accelerating growth factor secretion.

Keyword

Bone marrow cells; Fibroblasts; Growth substances; Wound healing

MeSH Terms

Bone Marrow Cells
Bone Marrow*
Culture Media
Fibroblasts*
Growth Substances
Humans
Mesenchymal Stromal Cells*
Pilot Projects
Transforming Growth Factor beta
Transforming Growth Factors
Vascular Endothelial Growth Factor A
Wound Healing*
Wounds and Injuries*
Culture Media
Growth Substances
Transforming Growth Factor beta
Transforming Growth Factors
Vascular Endothelial Growth Factor A