Korean J Urol.  1993 Feb;34(1):1-6.

Expression of brdU in BBN induced rat bladder tumor

• Kim WJ
• Song HG
• Kim YK

Abstract

We labeled-S-phase cell in BBN induced rat bladder tumors using anti-bromodeoxyuridine anti- body. The thymidine analogue, bromodeoyuridine (BrdU), was incorporated into DNA during the S -phase or the cell cycle. Anti-BrdU monoclonal antibody can be detected by the immunohistochemical method for an in vivo labeling study. Sequential cellular change (normal, simple hyperplasia, nodular or papillary hyperplasia, and transitional cell carcinoma-Ta, T1) was observed in rat urinary bladder according to the duration of BBN administration. The BrdU labeling index, S phase fraction, was determined by counting the number of BrdU labeled cells in the tissue section. The average labeling index obtained using this method in normal, simple hyperplasia(SH), nodular or papillary hyperplasia(NPH), and bladder tumor (Ta. T1) was 1.7, 5.6, 11.2, and 18.9 (14.9, 21.9), respectively. Also, the higher S phase fraction was found in grade II than in grade I .The higher S phase fraction indicated greater biological malignancy based on the fact that mean labeling index progressively increased with tumorigenesis. However, mean labeling index at NPH did not significantly differ from that of Ta, which implies that NPH might represent the similar cellular characteristics.

Keyword

BBN; Bladder tumor; Bomodeoxyuridine (BrdU); Labeling index

MeSH Terms

Animals
Bromodeoxyuridine*
Carcinogenesis
Cell Cycle
DNA
Hyperplasia
Rats*
S Phase
Thymidine
Urinary Bladder Neoplasms*
Urinary Bladder*
Bromodeoxyuridine
DNA
Thymidine