A Study of Relationship between Exposure to Manganese Chloride and Malondialdehyde in Rat Tissues

Moon, Chul Jin; Lee, Soo Jin; Lee, Se Hoon

Abstract

OBJECTIVES
This research was intended to investigate the relationship between manganese and malodndialdehyde concentration in tissues of rats exposed to maganese chloride.
METHODS
The study groups were 12 manganese treated rats and 9 control rats. Manganese treated rats were given intraperitoneally manganese chloride (Mn, 4 mg/kg) daily for a period of 30 days except Sunday. Control rats were injected 1ml of saline. The plasma manganese concentrations of rats were determined by graphite furnace atomic absorption spectrometry. The tissue manganese concentration was determined by flame atomic absorption spectrometry. Malondialdehyde, the product of lipid peroxidation was determined by ultraviolet-visible spectrophotometry. The plasma malondialdehyde was determined by gas chromatography with mass-detector. Protein concentration was quantified by ultraviolet-visible spectrometry and was used for the compensation of tissue malondialdehyde and manganese concentration.
RESULTS
Manganese concentrations of plasma, brain, liver, and pancreas were very significantly higher in the manganese-treated rats than in the control rats. Malondialdehyde concentration of plasma, brain, and pacrease were significantly higher in the manganese-treated rats than in the control rats. The concentration of malondialdehyde was correlated with manganese levels in plasma, brain and pancreas.
Conclusion
Based on the results obtained as above, it was concluded that the malondialdehyde, product of lipid peroxidation was related to the cell death due to dosing excess manganese.