J Korean Neurosurg Soc.  1992 Jan;21(1):23-29.

Loss of Heterozygosity of Chromosome 17p in Brain Tumors

• Baek SH
• Kim ES
• Kim DG
• Jung HW
• Kim HJ
• Cho BK
• Han DH
• Lee SH
• Kim JH

Abstract

With the methods of restriction fragment length polymorphisms(RFLPs) and southern blot analysis, gene deletion of chromosome 17p in 16 cases of brain tumors, was investigated. There were 4 cases of glioblastoma multiforme, 1 case of anaplastic astrocytoma, 4 cases of low grade astrocytoma, 3 cases of oligodendroglioma, and 4 cases of meningioma. Among restriction fragment length polymorphism(RFLP) DNA located in chromosome 17p, p144D 6 and p SNZ 22 were imployed as the probes. In eight of 16 cases(50%) constitutional heterozygosity was observe dfor p144 D6 probe on the short arm of chromosome 17, and in nine of 16 cases(56%) for PYNZ 22.1 probe. With both probes constitutional heterozygosity was observed in thirteen of 16 cases(81%). And the loss of constitutional heterozygosity was detected in two of 14 informative cases. Although, with the malignant gliomas, including 4 cases of glioblastoma multiforme and 1 case of anaplastic astrocytoma, two of 4 informative cases showed loss of constitutional heterozygosty, None of 9 informative cases showed loss of heterozygosity with the other brain tumors(low grade astrocytoma, oligodendroglioma, and meningioma).

Keyword

RFLP; Southern blot analysis; Glioblastoma multiforme; Anaplastic astrocytoma; Loss of heterozygosity

MeSH Terms

Arm
Astrocytoma
Blotting, Southern
Brain Neoplasms*
Brain*
Chromosomes, Human, Pair 17
DNA
Gene Deletion
Glioblastoma
Glioma
Loss of Heterozygosity*
Meningioma
Oligodendroglioma
Polymorphism, Restriction Fragment Length
DNA