J Korean Acad Periodontol.  1998 Jun;28(2):291-308. 10.5051/jkape.1998.28.2.291.

EFFECTS OF EXTRACTS OF DRYNARIAE RHIZOMA ON THE CHARACTERISTICS OF RAT CALARIA AND BONE MARROW CELLS

• Lim KS
• Kwon YH
• Park JB
• Kim SJ
• Choung SY
• Park KK

• KMID: 2014680
• DOI: http://doi.org/10.5051/jkape.1998.28.2.291

Abstract

This study was performed to evaluate the effects of extracts of Drynariae Rhizoma on the characteristics of rat calvaria cells(RCV) and bone marrow cells(RBM) which have the important role on the bone formation in vitro. Drynariae Rhizoma has been known as the useful herbal medicament for treatment of the wound healing including regeneration of bone fracture, and also has been used to treat the periodontal lesions, tooth mobility, gingival bleeding and pus discharge via sulcus in Oriental Medicine. In control group, the cells were cultured alone with Dulbeco's Modified Eagle's Medium contained with 10% fetal bovine serum, 100U/ml penicillin, 100microgram/ml streptomycin, 0.5microgram/ml amphotericin-B. In experimental group, extracts of Drynariae Rhizoma(0.1, 1, 5, 10, 50microgram/ml) were added into the above culture condition. And then each group was characterized by examing the cell proliferation at 1, 3, 7, 14, 21, 30th day, the amount of total protein synthesis and alkaline phosphatase activity of RCV at 2,4th day and those of RBM at 3, 6th day. And also, the calcified nodule of RCV was examed at 3, 5th day in three goup, control, experimental, culture with the PDGF group. The results were as follow; 1. Both RCV and RBM cells in Drynariae Rhizoma-treated experimental group proliferated more rapidly than non-treated control group. The experimental group below 5microgram/ml Drynariae Rhizoma-treated showed more prominent cell proliferation from the 7th day to the 21st day than the control group and above 10 microgram/ml treated group in RCV. 2. Amount of total protein synthesis was more increased in Drynariae Rhizoma-treated group than in control group. In 5microgram/ml Drynariae Rhizoma-treated group showed most prominent protein synthesis of the any other exrperimental group and control group. 3. Alkaline phosphatase activity also more increased in Drynariae Rhizoma-treated group than control group.

MeSH Terms

Alkaline Phosphatase
Animals
Bone Marrow Cells*
Bone Marrow*
Cell Proliferation
Fractures, Bone
Hemorrhage
Medicine, East Asian Traditional
Osteogenesis
Penicillins
Polypodiaceae*
Rats*
Regeneration
Skull
Streptomycin
Suppuration
Tooth Mobility
Wound Healing
Alkaline Phosphatase
Penicillins
Streptomycin