Tuberc Respir Dis.  2005 Mar;58(3):276-284. 10.4046/trd.2005.58.3.276.

Protective Effect of the Inhibition of PAF Remodeling and Adhesion Molecule on the Oxidative Stress of the Lungs of Rats Given Endotoxin Intratracheally

• Shin TR
• Na BK
• Lee YM

• KMID: 2009836
• DOI: http://doi.org/10.4046/trd.2005.58.3.276

Abstract

BACKGROUND: There have been many reports on the pathogenesis of sepsis-induced acute respiratory distress syndrome(ARDS) but, the precise mechanism has not been elucidated. This study examined the protective effect of an inhibition of platelet activating factor(PAF) remodeling and the adhesion molecule on the oxidative stress of the lungs in rats with an endotoxin induced acute lung injury(ALI).
METHODS
ALI was induced in Sprague-Dawley rats by instilling an E-coli endotoxin into the trachea. Ketotifen and fucoidan were used respectively to inhibit PAF remodeling and adhesion molecule. The lung leak index, lung myeloperoxidase(MPO) activity, bronchoalveolar lavage(BAL) fluid neutrophil count and lyso PAF acetyltransferase activity(AT), were measured and an ultrastructural study and cytochemical electron microscopy were performed.
RESULTS
The lung leak index, lung MPO activity, BAL fluid neutrophil count and lyso PAF AT activity was higher in the endotoxin-treated rats. In addition, severe destruction of the pulmonary architecture and increased hydrogen peroxide production were identified. These changes were reversed by ketotifen. However, fucoidan did not appear to have any protective effects.
CONCLUSION
The inhibition of PAF remodeling appeared to be effective in decreasing the endotoxin-induced ALI. In addition, this effect might be derived from the inhibition of neutrophilic oxidative stress. However, the inhibition of the adhesion molecules by fucoidan appeared to be ineffective in decreasing the endotoxin-induced ALI.

Keyword

ALI; PAF remodelling; Adhesion molecule; Oxidative stress

MeSH Terms

Animals
Blood Platelets
Hydrogen Peroxide
Ketotifen
Lung*
Microscopy, Electron
Neutrophils
Oxidative Stress*
Rats*
Rats, Sprague-Dawley
Trachea
Hydrogen Peroxide
Ketotifen