J Korean Surg Soc.  2003 Jul;65(1):35-41.

The Immunohistochemical Expression of E2F-1 Protein and DNA Topoisomerase II-alpha E2F-1 Protein in Colorectal Cancer and Their Relationship with Clinicopathologic Factors

• Hwang SI
• Lee TJ
• Park YG
• Ji GC
• Lee JH
• Chang IT
• Park SI

Abstract

PURPOSE
E2F-1 is a transcriptor that converts G1 to S in the cell cycle, and Topoisomerase II-alpha is a key enzyme in the metabolism of DNA, and an indicator of cell replication. The purpose of this study was to evaluate the clinical validity of E2F-1 and Topoisomerase II-alpha as prognostic factors in colorectal cancer. METHODS: The expressions of E2F-1 and Topoisomerase II-alpha were studied immunohistochemically using tumor specimen sections fixed with formalin and paraffin-embedded for 84 cases of colorectal cancer. The correlation between E2F-1 and Topoisomerase II-alpha expressions, and their relationship with the clinicopathological factors, such as tumor differentiation, tumor invasion, lymph node metastasis and tumor stage were investigated. RESULTS: Of the 84 specimens, 43 (51.2%) were immunohistochemically negative for E2F-1, and 41 (48.8%) were positive. The expression of E2F-1 correlated with poor tumor differentiation, increased lymph node metastasis and high tumor stage. The expression of Topoisomerase II-alpha also correlated with poor tumor differentiation, increased lymph node metastasis and high tumor stage. The E2F-1 and Topoisomerase II-alpha expressions indices were significantly correlated. CONCLUSION: These results suggest that the expressions of E2F-1 and DNA Topoisomerase II-alpha may play a role as a prognostic factor for colorectal cancer, but further studies will be required for its comfirmation.

Keyword

Colorectal cancer; E2F-1; Topoisomerase II-alpha

MeSH Terms

Cell Cycle
Colorectal Neoplasms*
DNA
DNA Topoisomerases, Type I*
Formaldehyde
Lymph Nodes
Metabolism
Neoplasm Metastasis
DNA
DNA Topoisomerases, Type I
Formaldehyde