J Korean Acad Periodontol.  2005 Jun;35(2):263-269. 10.5051/jkape.2005.35.2.263.

Effect of the combined use of bone morphogenetic protein and platelet-derived growth factor on bone formation in nude mouse

• Lee SH
• Choi BH
• Zhu SJ
• Huh JY
• Jung JH
• Kim BY

• KMID: 1999755
• DOI: http://doi.org/10.5051/jkape.2005.35.2.263

Abstract

Bone morphogenetic protein(BMP) and platelet-derived growth factor(PDGF) have been demonstrated tostimulate bone formation when applied locally in vivo. To explore whether or not the combined use of BMP and PDGF could have promotive effect and synergic interac- tion on bone formation in vivo, bone marrow mesenchymal stem cells were treated with BMP-2, PDGF-BB, or BMP-2 plus PDGF-BB, and then these cells were injected into the subcutaneous space on the dorsum of nude mice. The bone formation was evaluated after 12 weeks. Histomorphometric analysis demonstrated that the subcutaneous nodules formed in nude mice contained 25.3% newly formed bone in the BMP-2 treated cells, 14.4% newly formed bone in the PDGF-BB treated cells, and 8.9% newly formed bone in the BMP-2 plus PDGF-BB treated cells. The results showed that the combination of BMP-2 and PDGF-BB had neither a promotive effect nor synergic interact on bone formation in vivo.

Keyword

BMP; PDGF; growth factor; bone formation

MeSH Terms

Animals
Bone Marrow
Bone Morphogenetic Proteins*
Mesenchymal Stromal Cells
Mice
Mice, Nude*
Osteogenesis*
Platelet-Derived Growth Factor*
Bone Morphogenetic Proteins
Platelet-Derived Growth Factor