Korean J Urol.  2007 Mar;48(3):304-309. 10.4111/kju.2007.48.3.304.

Detection of Cryptic Microorganisms in Patients with Chronic Prostatitis by Multiplex Polymerase Chain Reaction

• Kim TH
• Kim TH
• Kim HR
• Lee MK
• Myung SC
• Kim YS

• KMID: 1997129
• DOI: http://doi.org/10.4111/kju.2007.48.3.304

Abstract

PURPOSE
Chronic prostatitis frequently occurs in men of all ages. Recent studies suggest that fastidious microorganisms may play a role in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). The aim of this study was to evaluate the usefulness and significance of multiplex polymerase chain reaction (PCR) in the diagnosis of CP/CPPS.
MATERIALS AND METHODS
First voided urine (FVU) and/or expressed prostatic secretions (EPS) were collected from 92 patients. Multiplex PCR, using Dual Specificity Oligo (DSO(TM)) primers, was used to test for Chlamydia trachomatis (CT), Mycoplasma genitalium (MG), Mycoplasma hominis (MH), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV) and Ureaplasma urealyticum (UU).
RESULTS
Multiplex PCR can be easily analyzed via visual comparison. Nine (39.1%) of the 23 CP/CPPS IIIa and 12 (17.4%) of the 69 IIIb patients had positive multiplex PCR, with a total of 27 microorganisms isolated, including CT, MH, MG, UU, TV and NG in 9, 7, 4, 4, 2 and 1 case, respectively. Co-infections with 2 or 3 organisms occurred in 5 cases. For the samples collected from 32 patients for both FVU and EPS, 68.7% gave the same results.
CONCLUSIONS
Multiplex PCR, using DSO(TM) primers, can be useful for the simple detection of fastidious microorganisms in CP/CPPS. To achieve reliable results with multiplex PCR, feasible guidelines and standardization are of major importance. Further studies will be required to define the usefulness of molecular tests for CP/CPPS in clinical practice.

Keyword

Prostatitis; Polymerase chain reaction

MeSH Terms

Chlamydia trachomatis
Coinfection
Diagnosis
Humans
Male
Multiplex Polymerase Chain Reaction*
Mycoplasma genitalium
Mycoplasma hominis
Neisseria gonorrhoeae
Pelvic Pain
Polymerase Chain Reaction
Prostatitis*
Sensitivity and Specificity
Trichomonas vaginalis
Ureaplasma urealyticum

Figure

• 

  Fig. 1 Species-specific DNA amplifications by multiplex polymerase chain reaction (PCR) in clinical specimens. Lane M, Molecular size marker; lane 1 (348bp), Chlamydia trachomatis; lane 2 (253bp), Mycoplasma genitalium; lane 3 (502bp), Mycoplasma hominis; lane 4 (214bp), Neisseria gonorrhoeae; lane 5 (580bp), Trichomonas vaginalis; lane 6 (435bp), Ureaplasma urealyticum; lane 7 (348, 435 and 502bp), positive control for Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis; lane 8, negative control. Internal control bands are indicated by an arrow (719bp).

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