Korean J Obstet Gynecol.  2012 Apr;55(4):244-249. 10.5468/KJOG.2012.55.4.244.

Intra-peritoneal NF-kappaB decoy oligodeoxynucleotide decreases postoperative intra-abdominal adhesion

• Ahn BN
• Song MH
• Kim JH
• Kim KH
• Park KK
• Choi YS

• KMID: 1992620
• DOI: http://doi.org/10.5468/KJOG.2012.55.4.244

Abstract

OBJECTIVE
The objective of this study was to evaluate the effect of intra-peritoneal nuclear factor kappaB (NF-kappaB) decoy oligodeoxynucleotide on the prevention of postoperative intra-abdominal adhesion.
METHODS
Forty female BALB/c mice were randomly assigned into 2 groups. The mice were anesthetized and then midline abdominal incision was made. Peritoneal injury was given using cytobrush until deperitonealization. The experimental group (n = 20) received NF-kappaB decoy oligodeoxynucleotide (NF-kappaB decoy ODN) in the peritoneal cavity before abdominal closure, while the control group (n = 20) received saline. At 2 weeks postoperation, after the mice were euthanized, the status of intra-abdominal adhesion was evaluated. The degrees of adhesion were graded into no (grade 0), mild (grade 1), moderate (grade 2), and severe adhesion (grade 3).
RESULTS
Two mice in the experimental group and 3 mice in the control group were dead within 1 day postoperation. In the control group, 2, 2, and 13 mice showed mild, moderate, and severe adhesion, respectively. In the experimental group, 4 mice showed no adhesion, whereas, 6 and 8 mice showed mild and moderate adhesion, respectively. The difference of degrees of adhesion between the groups was statistically significant (P <0.001).
CONCLUSION
Intra-peritoneal NF-kappaB decoy ODN reduced the severity of postoperative intra-abdominal adhesion.

Keyword

Adhesion; Inflammation; NF-kappa B; NF-kappa B decoy oligodeoxynucleotide

MeSH Terms

Animals
Female
Humans
Inflammation
Mice
NF-kappa B
Oligodeoxyribonucleotides
Peritoneal Cavity
NF-kappa B
Oligodeoxyribonucleotides

Figure

• 

  Fig. 1 Structure of ring type-nuclear factor -κB (NF-κB) decoy oligodeoxynucleotide (From Kim et al. [23] with permission from Elsevier).

• 

  Fig. 2 The representatives of the each grade of intra-abdominal adhesion severity. (A) No adhesion (grade 0). (B) Mild adhesion (grade 1). (C) Moderate adhesion (grade 2). (D) Severe adhesion (grade 3).

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