Korean J Vet Res.  2011 Sep;51(3):209-216.

Isolation and characterization of fowl adenovirus serotype 4 from chickens with hydropericardium syndrome in Korea

• Park HS
• Lim IS
• Kim SK
• Kim TK
• Yeo SG

Abstract

Four strains of fowl adenovirus (FAdV) were isolated from 4 flocks of broiler or layer chickens affected by hydropericardium syndrome in Korea. These FAdVs were classified as serotype 4 by restriction fragment length polymorphism patterns of hexon genes and whole genomes. The virus exhibited cytopathic effects consisting of rounding, ballooning and clustering in primary chicken embryo liver cell cultures. In transmission electron microscopy, virus particles in hexagonal shape aggregated exclusively in the nuclei of hepatocytes of the chickens as the typical appearances in adenovirus infections. Buoyant density of the virus in cesium chloride (CsCl) was 1.34 g/mL. The virus was stable to chloroform, ether, 50~70% ethanol, acidic condition at pH 3, 0.25% trypsin (1 : 250), heat at 50degrees C for 30 min, but labile to 100% ethanol, heat at 52~60degrees C for 30 min, 1 M MgCl2 at 50degrees C for 1 h, 1 : 2,000 formalin (37%). All of the physicochemical properties pertained to the characteristics of adenoviruses. Eight viral polypeptides were determined in CsCl-purified virus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Keyword

adenovirus properties; fowl adenovirus; polypeptides; RFLP patterns

MeSH Terms

Adenoviridae
Adenoviridae Infections
Cell Culture Techniques
Cesium
Chickens
Chlorides
Chloroform
Electrophoresis
Embryonic Structures
Ethanol
Ether, Ethyl
Formaldehyde
Genome
Hepatocytes
Hot Temperature
Hydrogen-Ion Concentration
Korea
Liver
Magnesium Chloride
Microscopy, Electron, Transmission
Peptides
Polymorphism, Restriction Fragment Length
Sodium
Trypsin
Virion
Viruses
Cesium
Chlorides
Chloroform
Ethanol
Ether, Ethyl
Formaldehyde
Magnesium Chloride
Peptides
Sodium
Trypsin