Mycobiology.  2005 Dec;33(4):188-193.

Isolation and Characterization of Cryptococcus neoformans from Environmental Sources in Busan

Affiliations
  • 1Maritime Safety Team, Korea Institute of Maritime and Fisheries Technology, Busan 608-080, Korea.
  • 2Department of Clinical Laboratory Science, Catholic University of Pusan, Busan 609-757, Korea. smhwang@cup.ac.kr

Abstract

Twenty nine samples of pigeon droppings (n = 12) and soil contaminated with avian excreta (n = 19), collected from different sites in Busan, were examined for isolation and characterization of Cryptococcus neoformans. Of these samples, 5 strains of C. neoformans were recovered from pigeon droppings (5/12 : 41.7%). All isolates were belonged to C. neoformans var. grubii (serotype A). The extracellular enzyme activities of the strains by using the API-ZYM system showed two different enzymatic patterns. The genetic variability among C. neoformans isolates was analyzed by random amplified polymorphic DNA (RAPD) using three 10-mer primers. Two different RAPD patterns, which clearly distinguished the isolates, were identified. Analysis of RAPD patterns provided a good characterization of environmental strains of C. neoformans serotype A as a heterogeneous group and were in good agreement with enzymatic profiles.

Keyword

API-ZYM; Cryptococcus neoformans; Pigeon droppings; RAPD

MeSH Terms

Busan*
Columbidae
Cryptococcus neoformans*
Cryptococcus*
DNA
Soil
DNA
Soil
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