Mycobiology.
2005 Dec;33(4):188-193.
Isolation and Characterization of Cryptococcus neoformans from Environmental Sources in Busan
- Affiliations
-
- 1Maritime Safety Team, Korea Institute of Maritime and Fisheries Technology, Busan 608-080, Korea.
- 2Department of Clinical Laboratory Science, Catholic University of Pusan, Busan 609-757, Korea. smhwang@cup.ac.kr
Abstract
- Twenty nine samples of pigeon droppings (n = 12) and soil contaminated with avian excreta (n = 19), collected from different sites in Busan, were examined for isolation and characterization of Cryptococcus neoformans. Of these samples, 5 strains of C. neoformans were recovered from pigeon droppings (5/12 : 41.7%). All isolates were belonged to C. neoformans var. grubii (serotype A). The extracellular enzyme activities of the strains by using the API-ZYM system showed two different enzymatic patterns. The genetic variability among C. neoformans isolates was analyzed by random amplified polymorphic DNA (RAPD) using three 10-mer primers. Two different RAPD patterns, which clearly distinguished the isolates, were identified. Analysis of RAPD patterns provided a good characterization of environmental strains of C. neoformans serotype A as a heterogeneous group and were in good agreement with enzymatic profiles.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Molecular Characterization of Clinical and Environmental Strains of Cryptococcus neoformans Isolated from Busan, Korea
- Genotypes of Clinical and Environmental Isolates of Cryptococcus neoformans and Cryptococcus gattii in Korea
- Isolation of Cryptococcus neoformans var. grubii (serotype A) from Pigeon Droppings in Korea
- In vitro Evaluation of the Antifungal Activity of Propolis Extract on Cryptococcus neoformans and Candida albicans
- Identification and Functional Characterization of a Cryptococcus neoformans UPC2 Homolog
