Korean J Thorac Cardiovasc Surg.
2008 Feb;41(1):25-33.
The Role of GADD45beta in Acute Myocardial Injury
- Affiliations
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- 1Department of Thoracic and Cardiovascular Surgery, Kyungpook National University Hospital, College of Medicine, Kyungpook National University, Korea.
- 2Department of Thoracic and Cardiovascular Surgery, Chungbuk University Hospital, College of Medicine, Chungbuk National University, Korea.
- 3Department of Thoracic and Cardiovascular Surgery, Seoul National University Children's Hospital, Seoul National University College of Medicine, Korea. jrl@plaza.snu.ac.kr
- 4Xenotransplantation Research Center, Clinical Research Institute, Seoul National University Hospital, Korea.
- 5Department of Cardiothoracic Surgery, Hallym University Sacred Heart Hospital, College of Medicine, Hallym University, Korea.
Abstract
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BACKGROUND: A critical shortage of donor organs has necessitated an investigation of new strategies to increase the availability of additional organs available for human transplantation. We investigated the amount of apoptosis and expression of GADD45beta in two groups, a GADD45beta-transfected group and untransfected group.
MATERIAL AND METHOD: The experimental groups consist of a control group (normal H9C2 cell line) and GADD45beta-transfected group. After injury of the each group, we evaluated the expression of GADD45beta and the level of apoptosis in each group.
RESULT: There was a significant increase in the expression of GADD45beta in the GADD45beta-transfected group at 1 hour, 2 hours, and 3 hours after stimuli as compared with the control group. The amount of cardiac myoblast cell line apoptosis was significantly lower in the GADD45beta-transfected group as compared with the control group. The concentration of annexin in the GADD45beta-transfected group was significantly lower than that of the control group after cell injury.
CONCLUSION
Transfection of a rat myoblast cell line with the GADD45beta gene results in decreased susceptibility to cell injury of human serum.
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