J Korean Acad Conserv Dent.  2006 Jul;31(4):290-299. 10.5395/JKACD.2006.31.4.290.

The effect of ethylene glycol analogs on mechanical properties of moist demineralized dentin matrix

• Lee KH
• Cho YG
• Lee KW

• KMID: 1986872
• DOI: http://doi.org/10.5395/JKACD.2006.31.4.290

Abstract

OBJECTIVES
The purpose of this study is to evaluate the effect of ethylene glycol analogs on modulus of elasticity and ultimate tensile strength of moist, demineralized dentin matrix.
METHODS
Dentin disks 0.5 mm thick were prepared from mid-coronal dentin of extracted, unerupted, human third molars. "I" beam and hour-glass shaped specimens were prepared from the disks, the ends protected with nail varnish and the central regions completely demineralized in 0.5M EDTA for 5 days. Ultimate tensile stress (UTS) and low strain modulus of elasticity (E) were determined with specimens immersed for 60 min in distilled water (H2O), ethylene glycol (HO-CH2-CH2-OH), 2-methoxyethanol (H3CO-CH2-CH2-OH), and 1,2-dimethoxyethane (H3CO-CH2-CH3-OCH3) prior to testing in those same media. Modulus of elasticity was measured on the same specimens in a repeated measures experimental design. The results were analyzed with a one-way ANOVA on ranks, followed by Dunn's test at alpha = 0.05. Regression analysis examined the relationship between UTS or E and hoy's solubility parameter for hydrogen bonding (delta(h)) of each solvent.
RESULTS
The UTS of demineralized dentin in water, ethylene glycol, 2-methoxyethanol, and 1,2-dimethoxyethane was 24 (3), 30 (5), 37 (6), and 45 (6) MPa, x (SD) N = 10. Low strain E for the same media were 16 (13), 23 (14), 52 (24), and 62 (22) MPa. Regression analysis of UTS vs delta(h) revealed a significant (p < 0.0001, r = -0.99, R2 = 0.98) inverse, exponential relationship. A similar inverse relationship was obtained between low strain E vs delta(h) (p < 0.0005, r = -0.93, R2 = 0.86). SIGNIFICANCE: The tensile properties of demineralized dentin are dependent upon the hydrogen bonding ability of polar solvents (delta(h)). Solvents with low delta(h) values may permit new interpeptide H-bonding in collagen that increases its tensile properties. Solvents with high delta(h) values prevent the development of these new interpeptide H-bonds.

Keyword

Polar Solvents; Ethylene Glycol Analogs; Hydrogen Bonding Ability

MeSH Terms

Collagen
Dentin*
Edetic Acid
Elastic Modulus
Ethylene Glycol*
Humans
Hydrogen Bonding
Molar, Third
Paint
Research Design
Solubility
Solvents
Sprains and Strains
Tensile Strength
Water
Collagen
Edetic Acid
Ethylene Glycol
Solvents
Water

Figure

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  Figure 1 Schematic of sample preparation from mid-coronal dentin disk 0.5 mm thick (A). Hour-glass (B) and "I" beam (C) specimens were cut from the discs.

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  Figure 2 Two dimensional chemical structures of the control and test solvents.

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  Figure 3 Illustration of how the demineralized "I" beam was placed into friction grips of a universal testing machine.

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  Figure 4 Split-aluminum mold to measure the ultimate tensile strength by placing hourglass pattern into depressions.

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  Figure 5 UTS Values of Polar Solvents in 0.5 M EDTA Demineralized Dentin.

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  Figure 6 Hoy's Solubility Parameter vs UTS of Demineralized Dentin Matrix.

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  Figure 7 Hoy's Solubility Parameter vs UTS of Demineralized Dentin Matrix.

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  Figure 8 Low Wtrain Modulus of Elasticity of Polar Solvents.

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  Figure 9 Hoy's Solubility Parameter vs Low Strain Modulus of Elasticity.

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