Korean J Physiol Pharmacol.  2000 Apr;4(2):121-127.

The role of lipid peroxidation and glutathione on the glycochenodeoxycholic acid-induced cell death in primary cultured rat hepatocytes

Affiliations
  • 1Department of Pharmacology, College of Medicine, Ewha Womans University, 911-1 Mok-6-dong, Yangchon-gu, Seoul, South Korea.

Abstract

Intracellular accumulation of bile acids in the hepatocytes during cholestasis is thought to be pathogenic in cholestatic liver diseases. The objective of this study was to determine the role of lipid peroxidation and glutathione on the bile acid-induced hepatic cell death mechanism in primary cultured rat hepatocytes. To induce hepatic cell death, we incubated primary cultured rat hepatocytes with glycochenodeoxycholic acid (GCDC; 0~400 micrometer) for 3 hours. In electron microscopic examination and agarose gel electrophoresis, low concentration of GCDC treatment mainly induced apoptotic feature. Whereas 400 micrometer GCDC treated cells demonstrated both apoptosis and necrosis. Lipid peroxidation was increased dose-dependently in GCDC treated hepatocyte. And this was also accompanied by decreased glutathione. Therefore, oxygen free radical damage may play a partial role in GCDC-induced hepatic cell death.

Keyword

Hepatocyte; Apoptosis; Glycochenodeoxycholic acid; Lipid peroxidation; Glutathione

MeSH Terms

Animals
Apoptosis
Bile
Bile Acids and Salts
Cell Death*
Cholestasis
Electrophoresis, Agar Gel
Glutathione*
Glycochenodeoxycholic Acid
Hepatocytes*
Lipid Peroxidation*
Liver Diseases
Necrosis
Oxygen
Rats*
Bile Acids and Salts
Glutathione
Glycochenodeoxycholic Acid
Oxygen
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