Korean J Physiol Pharmacol.
2000 Apr;4(2):121-127.
The role of lipid peroxidation and glutathione on the
glycochenodeoxycholic acid-induced cell death in primary cultured rat
hepatocytes
- Affiliations
-
- 1Department of Pharmacology, College of Medicine, Ewha Womans
University, 911-1 Mok-6-dong, Yangchon-gu, Seoul, South Korea.
Abstract
- Intracellular accumulation of bile acids in the hepatocytes during
cholestasis is thought to be pathogenic in cholestatic liver diseases.
The objective of this study was to determine the role of lipid
peroxidation and glutathione on the bile acid-induced hepatic cell
death mechanism in primary cultured rat hepatocytes. To induce hepatic
cell death, we incubated primary cultured rat hepatocytes with
glycochenodeoxycholic acid (GCDC; 0~400 micrometer) for 3 hours. In
electron microscopic examination and agarose gel electrophoresis, low
concentration of GCDC treatment mainly induced apoptotic feature.
Whereas 400 micrometer GCDC treated cells demonstrated both apoptosis and
necrosis. Lipid peroxidation was increased dose-dependently in GCDC
treated hepatocyte. And this was also accompanied by decreased
glutathione. Therefore, oxygen free radical damage may play a partial
role in GCDC-induced hepatic cell death.