Korean J Phys Anthropol.  2008 Dec;21(4):381-390.

Immunohistochemical Study on the Distribution of Insulin-like Growth Factor Binding Protein 7 (IGFBP7) in the Central Nervous System of Adult Rats

• Lee HJ
• Ahn HS
• Park BJ
• Cho DY
• Chung YH
• Kim D
• Kim SS
• Kim KY
• Lee WB

Abstract

In the present study, we performed immunohistochemical studies to investigate the detailed distribution of insulin-like growth factor binding protein 7 (IGFBP7) in the central nervous system of adult rats. Twelve adult (4~6 month old) Sprague-Dawley rats were examined in this study. Immunohistochemistry using specific antibodies against IGFBP7 was performed in accordance with the free-floating method. In the present study, IGFBP7 immunoreactivity was observed in the cerebral cortex, hippocampus, brainstem, cerebellum and spinal cord. In the cerebral cortex, heavily stained neurons were seen in layers II-VI. In the hippocampus, pyramidal cells in CA1-3 region were strongly immunoreactive for IGFBP7. Strong immunoreactive neurons were also found in the supraoptic nucleus, paraventricular nucleus, periaqueductal gray and oculomotor nucleus. In the cerebellum, IGFBP7 immunoreactivity was prominent in the Purkinje cells and cerebellar output neurons. IGFBP7-immunoreactive neurons were prominent in the superior vestibular nucleus, cochlear nucleus, trigeminal motor nucleus, nucleus of the trapezoid, and facial nucleus. IGFBP7-immunoreactive neurons were also observed mainly in the anterior horn of the spinal cord. The first demonstration of IGFBP7 localization in the whole brain may provide useful data for the future investigations on the structural and functional properties of IGFBP7.

Keyword

Insulin-like growth factor binding protein 7 (IGFBP7); Cerebral cortex; Hippocampus; Brainstem; Cerebellum; Spinal cord

MeSH Terms

Adult
Animals
Antibodies
Brain
Brain Stem
Carrier Proteins
Central Nervous System
Cerebellum
Cerebral Cortex
Cochlear Nucleus
Hippocampus
Horns
Humans
Immunohistochemistry
Neurons
Paraventricular Hypothalamic Nucleus
Periaqueductal Gray
Purkinje Cells
Pyramidal Cells
Rats
Rats, Sprague-Dawley
Spinal Cord
Supraoptic Nucleus
Trigeminal Nuclei
Antibodies
Carrier Proteins