Korean J Fertil Steril.  2006 Dec;33(4):245-251.

Mitochondrial DNA Copy Number in the Patients of Korean Polycystic Ovary Syndrome (PCOS)

• Park JE
• Jang MH
• Cho SW
• Kim YS
• Won HJ
• Cho JH
• Baek KH
• Lee SH

Abstract

OBJECTIVE
We analyzed quantification of mitochondria DNA (mtDNA) to investigate the relationship of mitochondria and pathogenesis of PCOS.
MATERIALS AND METHODS
Peripheral blood samples were collected from 28 patients with PCOS who were under the inclusion criteria for PCOS and from 28 healthy controls. Genomic DNA was used to analyze real-time PCR for mtDNA copy number quantification. The mtDNA copy number was compared between the control and PCOS groups. All data was expressed as mean +/- SD. Statistical analysis was assessed by t-test.
RESULTS
In this study, the mtDNA CT was 11.67+/-0.422 in PCOS patients and 11.51+/-0.722 in control group, respectively. The mtDNA copy number was 1726410.71+/-407858.591 the patients of in PCOS and 2167887.51+/-252459.28 in control group (p=0.08), respectively.
CONCLUSION
In our study, using real-time PCR, there was a tendency of lower mtDNA copy number in the patients of PCOS when comparing to the control group even though statistical difference was not significant. However, more extensive analysis is required to clarity relationship between mtDNA copy number and pathogenesis of PCOS.

Keyword

PCOS; Mitochondria; Real-time PCR

MeSH Terms

DNA
DNA, Mitochondrial*
Humans
Mitochondria
Polycystic Ovary Syndrome*
Real-Time Polymerase Chain Reaction
DNA
DNA, Mitochondrial