Korean J Otolaryngol-Head Neck Surg.  1999 Nov;42(11):1405-1412.

Genetic Polymorphism of Cytochrome P4501A1 Exon 7 and Glutathione S-Transferase M1 in the Head and Neck Squamous Cell Carcinoma Patients

• Ko KM
• Ahn KS
• Tae K
• Lee SH
• Kong G

Abstract

BACKGROUND AND OBJECTIVES: An individual difference in susceptibility to chemically induced carcinomas is in part ascribed to genetic differences of metabolic activity of environmental procarcinogens. The cytochrome P450 family (CYPs) and glutathione S-transferase (GST) have been reported to be associated with human cancers related with smoking. The purpose of this study was to determine the frequencies of the genotypes of CYP1A1 and GSTM1 genes in healthy control of Koreans and to identify the high-risk genotypes of these metabolic genes in head and neck cancer patients. MATERIALS AND METHOD: The genetic polymorphism of CYP1A1 exon 7 and GSTM1 genes were analysed in a group of 115 healthy Koreans and 107 head and neck squamous cell carcinoma patients using allelic-specific polymerase chain reaction.
RESULTS
The genotypes of CYP1A1 exon 7 (Ile/Ile, Ile/Val and Val/Val) were 59.1%, 36.5% and 4.4%, respectively, in the healthy control group, and 57.0%, 31.8% and 11.2%, respectively, in the cancer patients . The distributions of GSTM1 [GSTM1 (-), GSTM1 (+)] in healthy control group were 46.1%, 53.9% respectively, and 53.3%, 46.7%, respectively, in the cancer patients. The relative risk (odds ratio) for combination of CYP1A1 Val/Val and GSTM1 (-) genotype was estimated to be 5.17, taking the risk of combined genotype Ile/Ile and GSTM1 (+) as a reference in cancer patients.
CONCLUSION
These results suggest that the genetic polymorphisms of CYP1A1 exon 7 and GSTM1 were an important major factor in determining the individual susceptibility to head and neck squamous cell carcinoma in Koreans.

Keyword

Genetic polymorphism; Head and neck cancer; Cytochrome P4501A1 exon 7; Glutathione S-Transferase M1

MeSH Terms

Carcinoma, Squamous Cell*
Cytochrome P-450 CYP1A1
Cytochrome P-450 Enzyme System
Cytochromes*
Exons*
Genotype
Glutathione Transferase*
Glutathione*
Head and Neck Neoplasms
Head*
Humans
Individuality
Neck*
Polymerase Chain Reaction
Polymorphism, Genetic*
Smoke
Smoking
Cytochrome P-450 CYP1A1
Cytochrome P-450 Enzyme System
Cytochromes
Glutathione
Glutathione Transferase
Smoke