Korean J Otolaryngol-Head Neck Surg.
2004 Sep;47(9):885-892.
Single Nucleotide Polymorphism Analysis in Larynx and Hypopharyx Squamous Cell Carcinoma
- Affiliations
-
- 1Department of Otolaryngology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea. sykim2@amc.seoul.kr
- 2Department of Radiation Oncology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
Abstract
- BACKGROUND AND OBJECTIVES
Although smoking and alcohol consumption are recognised as the major causes of head and neck cancer, profound regional differences remain unexplained. Genetic factors such as the single nucleotide polymorphism (SNP) alter the susceptibility of host to environmental factors. Therefore an aim of this study is to determine the effect of six genetic polymorphisms (Cytochrome P450 1B1 Arg48Gly, Cytochrome P450 1B1 Val432Leu, glutathione S-transferase P1 Ile105Val, ERCC1 Asn118Asn, p53 11827 (nt), p53 Arg72Pro) on the risk of head and neck cancer. SUBJECTS AND METHOD: Patients were diagnosed as larynx and hypopharynx squamous cell carcinoma between April 1992 and March 2003 at the Department of Otolaryngology, Asan Medical Center. 135 patients consists of 76 patients with glottic cancer, 33 patients with supraglottic cancer, and 26 patients with hypopharynx cancer and the mean age of patients was 63.5 years (range 35-83). The controls were cancer-free 123 individuals and the mean age was 51.3 years (range 22-73). Each subject donated 10ml of blood. B lymphocyte pallet was used for DNA extraction. We performed polymerase chain reaction (PCR) amplification. RESULTS: The allele frequencies of six polymorphisms were not statistically different between the cases and controls. CONCLUSION: We did not find any association between six polymorphisms and the risk for larynx and hypopahrynx squamous cell carcinoma in this case-control study. However, larger scale studies are needed to assess the effect of genetic polymorphism on the risk of larynx and hypopahrynx squamous cell carcinoma.
MeSH Terms
-
Alcohol Drinking
Carcinoma, Squamous Cell*
Case-Control Studies
Chungcheongnam-do
Cytochrome P-450 Enzyme System
DNA
Gene Frequency
Genes, p53
Glutathione Transferase
Head and Neck Neoplasms
Humans
Hypopharynx
Larynx*
Lymphocytes
Otolaryngology
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Single Nucleotide*
Smoke
Smoking
Cytochrome P-450 Enzyme System
DNA
Glutathione Transferase
Smoke
